Recombinant Anti-TMEM16A antibody [SP31] (ab64085)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP31] to TMEM16A
- Suitable for: IHC-P, Flow Cyt (Intra), ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TMEM16A antibody [SP31]
See all TMEM16A primary antibodies -
Description
Rabbit monoclonal [SP31] to TMEM16A -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Flow Cyt (Intra), ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cynomolgus monkey -
Immunogen
Synthetic peptide within Human TMEM16A aa 400-500. The exact sequence is proprietary.
Database link: Q5XXA6 -
Positive control
- IHC-P: Human GIST tumor, Human SBG from CF patients, Human surface epithelium tissue Flow Cyt (intra): PC-3 cells. WB: PC-3 whole cell lysate, Capan-1 whole cell lysate. ICC/IF: PC-3 (human prostate adenocarcinoma epithelial cell)
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General notes
ab64085 was switched from a hybridoma to recombinant production method on 23rd July 2019.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
SP31 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab64085 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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WB | (2) |
1/1000. Detects a band of approximately 130, 260 kDa (predicted molecular weight: 114 kDa).
PMID: 22685202. |
Notes |
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IHC-P
1/100. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
WB
1/1000. Detects a band of approximately 130, 260 kDa (predicted molecular weight: 114 kDa). PMID: 22685202. |
Target
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Function
Acts as a calcium-activated chloride channel. Required for normal tracheal development. -
Tissue specificity
Broadly expressed with higher levels in liver and skeletal muscle. -
Sequence similarities
Belongs to the anoctamin family. -
Domain
The region spanning the fifth and sixth transmembrane domains probably forms the pore-forming region. -
Cellular localization
Cell membrane. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 55107 Human
- Omim: 610108 Human
- SwissProt: Q5XXA6 Human
- Unigene: 503074 Human
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Alternative names
- ANO 1 antibody
- ANO1 antibody
- ANO1_HUMAN antibody
see all
Images
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ab64085 staining TMEM16A in PC-3 (human prostate adenocarcinoma epithelial cell) cells. The cells were fixed with 4% formaldehyde, permeabilized in 100% methanol. The cells were then incubated with ab64085 at 1/20 dilution, followed by secondary antibody ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used for counterstain at 1/200 dilution (Red). Nuclear DNA was labelled in blue with DAPI.
Confocal image showing membranous and cytoplasmic staining in PC-3 cell line.
Low expression control: LnCaP(PMID: 29899325)
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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All lanes : Anti-TMEM16A antibody [SP31] (ab64085) at 1/1000 dilution
Lane 1 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : Capan-1 (Human pancreas adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 114 kDa
Observed band size: 130,260 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time:
Lane 1: 20 seconds
Lane 2: 10 seconds
The observed molecular weights are consistent with PMID: 22685202.
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Intracellular Flow Cytometry analysis of PC-3 (Human prostate adenocarcinoma epithelial cell) cells labeling TMEM16A with purified ab64085. (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077) secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.
This image was generated from the hybridoma version.
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TMEM16A detected in paraffin-embedded sections of human submucosal glands from CF patients and control samples.
TMEM16A expression was modest in non-CF submucosal glands of non-CF samples (b) but markedly increased in tissues from CF patients (d), with a particularly strong signal (f) in histologically altered glands (e). Magnification: X630 in a, X400 in b-f. Images 4Aa and 4Ba,c,e show hematoxylin and eosin staining.
This image was generated from the hybridoma version.
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ab64085 showing expression of TMEM16A in human surface epithelium tissue. TMEM16A staining was mostly absent (b), and sometimes scanty (c) in the respiratory epithelium lining bronchi or bronchioles from CF patients (c, arrows). A weak expression was also detectable in microvessels of peri-bronchial connective tissue (b, arrows). Magnification: X400.
This image was generated from the hybridoma version.
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Immunohistochemical analysis of Human Gastrointestinal Stromal Tumor tissue labelling TMEM16A with ab64085.
This image was generated from the hybridoma version.
Datasheets and documents
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SDS download
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Datasheet download
References (49)
ab64085 has been referenced in 49 publications.
- Ousingsawat J et al. Expression of SLC26A9 in Airways and Its Potential Role in Asthma. Int J Mol Sci 23:N/A (2022). PubMed: 35328418
- Centeio R et al. CLCA1 Regulates Airway Mucus Production and Ion Secretion Through TMEM16A. Int J Mol Sci 22:N/A (2021). PubMed: 34066250
- Ousingsawat J et al. P. aeruginosa Induced Lipid Peroxidation Causes Ferroptotic Cell Death in Airways. Cell Physiol Biochem 55:590-604 (2021). PubMed: 34637202
- Cao X et al. Opposing roles of E3 ligases TRIM23 and TRIM21 in regulation of ion channel ANO1 protein levels. J Biol Chem 296:100738 (2021). PubMed: 33957127
- Choi MR et al. Anoctamin1 Induces Hyperproliferation of HaCaT Keratinocytes and Triggers Imiquimod-Induced Psoriasis-Like Skin Injury in Mice. Int J Mol Sci 22:N/A (2021). PubMed: 34281197