Recombinant Anti-TMEM16A antibody [SP31] - BSA and Azide free (ab198412)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP31] to TMEM16A - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TMEM16A antibody [SP31] - BSA and Azide free
See all TMEM16A primary antibodies -
Description
Rabbit monoclonal [SP31] to TMEM16A - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, Flow Cyt (Intra), WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cynomolgus monkey -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human GIST tumor, Human SBG from CF patients, Human surface epithelium tissue Flow Cyt (intra): PC-3 cells. WB: PC-3 whole cell lysate, Capan-1 whole cell lysate. ICC/IF: PC-3 (human prostate adenocarcinoma epithelial cell)
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General notes
ab198412 is the carrier-free version of ab64085.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
SP31 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab198412 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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WB |
1/1000. Detects a band of approximately 130, 260 kDa (predicted molecular weight: 114 kDa).
PMID: 22685202. |
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IHC-P |
1/100.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
WB
1/1000. Detects a band of approximately 130, 260 kDa (predicted molecular weight: 114 kDa). PMID: 22685202. |
IHC-P
1/100. |
Target
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Function
Acts as a calcium-activated chloride channel. Required for normal tracheal development. -
Tissue specificity
Broadly expressed with higher levels in liver and skeletal muscle. -
Sequence similarities
Belongs to the anoctamin family. -
Domain
The region spanning the fifth and sixth transmembrane domains probably forms the pore-forming region. -
Cellular localization
Cell membrane. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 55107 Human
- Omim: 610108 Human
- SwissProt: Q5XXA6 Human
- Unigene: 503074 Human
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Alternative names
- ANO 1 antibody
- ANO1 antibody
- ANO1_HUMAN antibody
see all
Images
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This data was developed using ab64085, the same antibody clone in a different buffer formulation.
ab64085 staining TMEM16A in PC-3 (human prostate adenocarcinoma epithelial cell) cells. The cells were fixed with 4% formaldehyde, permeabilized in 100% methanol. The cells were then incubated with ab64085 at 1/20 dilution, followed by secondary antibody ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used for counterstain at 1/200 dilution (Red). Nuclear DNA was labelled in blue with DAPI.
Confocal image showing membranous and cytoplasmic staining in PC-3 cell line.
Low expression control: LnCaP(PMID: 29899325)
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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All lanes : Anti-TMEM16A antibody [SP31] (ab64085) at 1/1000 dilution
Lane 1 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : Capan-1 (Human pancreas adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 114 kDa
Observed band size: 130,260 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time:
Lane 1: 20 seconds
Lane 2: 10 seconds
The observed molecular weights are consistent with PMID: 22685202.
This data was developed using ab64085, the same antibody clone in a different buffer formulation.
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Intracellular Flow Cytometry analysis of PC-3 (Human prostate adenocarcinoma epithelial cell) cells labeling TMEM16A with purified ab64085 at 1/20 dilution (11.3µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab64085).
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TMEM16A detected in paraffin-embedded sections of human submucosal glands from CF patients and control samples.
TMEM16A expression was modest in non-CF submucosal glands of non-CF samples (b) but markedly increased in tissues from CF patients (d), with a particularly strong signal (f) in histologically altered glands (e). Magnification: X630 in a, X400 in b-f. Images 4Aa and 4Ba,c,e show hematoxylin and eosin staining.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab64085).
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ab64085 showing expression of TMEM16A in human surface epithelium tissue. TMEM16A staining was mostly absent (b), and sometimes scanty (c) in the respiratory epithelium lining bronchi or bronchioles from CF patients (c, arrows). A weak expression was also detectable in microvessels of peri-bronchial connective tissue (b, arrows). Magnification: X400.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab64085).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab198412 has not yet been referenced specifically in any publications.