Anti-TMEM16B antibody (ab113443)

Reviews (3)Q&A (1)

Overview

  • Product name

    Anti-TMEM16B antibody
    See all TMEM16B primary antibodies

  • Description

    Rabbit polyclonal to TMEM16B

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ELISA, IHC-Pmore details

  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide from the N terminal region of Human TMEM16B, conjugated to KLH.

  • Positive control

    • Human small intestine and spleen tissues; Rat brain tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab113443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 114 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function

    Acts as a calcium-activated chloride channel (CaCC), mostly in photoreceptors. May mediate olfactory amplification in olfactory sensory neurons (OSNs) and light perception amplification in retina.

  • Tissue specificity

    Retina, especially in the photoreceptor synaptic terminals.

  • Sequence similarities

    Belongs to the anoctamin family.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Ano2 antibody
      • ANO2_HUMAN antibody
      • Anoctamin 2 antibody
      • Anoctamin-2 antibody
      • C12orf3 antibody
      • Chromosome 12 open reading frame 3 antibody
      • DKFZp434P102 antibody
      • Transmembrane protein 16B (eight membrane-spanning domains) antibody
      • Transmembrane protein 16B antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM16B antibody (ab113443)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM16B antibody (ab113443)
      ab113443, at 5 µg/ml, staining TMEM16B in formalin fixed, paraffin embedded Human small intestine tissue by Immunohistochemistry.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM16B antibody (ab113443)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM16B antibody (ab113443)
      ab113443, at 5 µg/ml, staining TMEM16B in formalin fixed, paraffin embedded Human spleen tissue by Immunohistochemistry.
    • Western blot - Anti-TMEM16B antibody (ab113443)
      Western blot - Anti-TMEM16B antibody (ab113443)
      Lane 1 : Anti-TMEM16B antibody (ab113443) at 1 µg/ml
      Lane 2 : Anti-TMEM16B antibody (ab113443) at 2 µg/ml

      All lanes : Rat brain tissue lysate

      Predicted band size: 114 kDa

    References

    ab113443 has not yet been referenced specifically in any publications.

    Publishing research using ab113443? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-TMEM16B antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: PBS/HCl/Borax
    Permeabilization
    Yes - 0.2% PBS-Tx
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 26°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 07 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TMEM16B antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Colon Ca)
    Specification
    Colon Ca
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 18 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TMEM16B antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (retina)
    Specification
    retina
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated
    Blocking step
    Zytomed Blocking Reagent as blocking agent for 30 minute(s) · Concentration: 0% · Temperature: 20°C
    Read More

    Ms. Andrea Dannullis

    Verified customer

    Submitted Oct 17 2012

    Question

    POSITIVE AND NEGATIVE CONTROLS USED negative control : GFP overexpression in CHO cell experiment : mAno2-GFP fusion protein overexpression in CHO cell

    Read More
    Answer

    The laboratory that produces ab113443 has sent the alignment of the immunogen with the rat and mouse proteins. As I mentioned in my previous e-mail, the identity of the immunogen sequence is considered proprietary. Homology between human and mouse looks like this: *****.:: :**.****** Homology between human and rat looks like this: *****.:: :**.***:** The differences between the immunogen and the homologous mouse sequence may account for the failure of the antibody to detect mAno2, but the effects of PFA fixation may also interfere with reactivity.

    Read More

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