Key features and details
- Rabbit polyclonal to TMEM16B
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-TMEM16B antibody
See all TMEM16B primary antibodies
DescriptionRabbit polyclonal to TMEM16B
SpecificityThis antibody will not cross-react with TMEM16A.
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human TMEM16B (N terminal).
Database link: Q9NQ90
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab91573 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 110 kDa.|
|IHC-P||Use a concentration of 5 µg/ml.|
FunctionActs as a calcium-activated chloride channel (CaCC), mostly in photoreceptors. May mediate olfactory amplification in olfactory sensory neurons (OSNs) and light perception amplification in retina.
Tissue specificityRetina, especially in the photoreceptor synaptic terminals.
Sequence similaritiesBelongs to the anoctamin family.
Cellular localizationCell membrane.
- Information by UniProt
- Ano2 antibody
- ANO2_HUMAN antibody
- Anoctamin 2 antibody
Immunohistochemical analysis of human brain tissue labelling TMEM16B with ab91573 at 5 ug/mL.
All lanes : Anti-TMEM16B antibody (ab91573) at 2 µg/ml
All lanes : Human small intestine tissue lysate
Predicted band size: 110 kDa
Observed band size: 107 kDa why is the actual band size different from the predicted?
Immunofluorescence of TMEM16B in Human Brain cells using ab91573 at 20 ug/ml.
Lane 1 : Anti-TMEM16B antibody (ab91573) at 1 µg/ml
Lane 2 : Anti-TMEM16B antibody (ab91573) at 2 µg/ml
All lanes : Rat brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 110 kDa
The lower molecular weight band may represent one of the 3 isoforms of this protein which are known to exist, or a posttranslational modification.
ab91573 has been referenced in 1 publication.
- Yamamura H et al. TMEM16A and TMEM16B channel proteins generate Ca2+-activated Cl- current and regulate melatonin secretion in rat pineal glands. J Biol Chem 293:995-1006 (2018). PubMed: 29187602