Overview

  • Product name

  • Description

    Rabbit polyclonal to TMEM16B
  • Host species

    Rabbit
  • Specificity

    This antibody will not cross-react with TMEM16A.
  • Tested applications

    Suitable for: ELISA, ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human TMEM16B (N terminal).
    Database link: Q9NQ90

  • Positive control

    • Rat brain tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab91573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 110 kDa.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function

    Acts as a calcium-activated chloride channel (CaCC), mostly in photoreceptors. May mediate olfactory amplification in olfactory sensory neurons (OSNs) and light perception amplification in retina.
  • Tissue specificity

    Retina, especially in the photoreceptor synaptic terminals.
  • Sequence similarities

    Belongs to the anoctamin family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Ano2 antibody
    • ANO2_HUMAN antibody
    • Anoctamin 2 antibody
    • Anoctamin-2 antibody
    • C12orf3 antibody
    • Chromosome 12 open reading frame 3 antibody
    • DKFZp434P102 antibody
    • Transmembrane protein 16B (eight membrane-spanning domains) antibody
    • Transmembrane protein 16B antibody
    see all

Images

  • Immunohistochemical analysis of human brain tissue labelling TMEM16B with ab91573 at 5 ug/mL.

  • All lanes : Anti-TMEM16B antibody (ab91573) at 2 µg/ml

    All lanes : Human small intestine tissue lysate

    Predicted band size: 110 kDa
    Observed band size: 107 kDa
    why is the actual band size different from the predicted?

  • Immunofluorescence of TMEM16B in Human Brain cells using ab91573 at 20 ug/ml.

  • Lane 1 : Anti-TMEM16B antibody (ab91573) at 1 µg/ml
    Lane 2 : Anti-TMEM16B antibody (ab91573) at 2 µg/ml

    All lanes : Rat brain tissue lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 110 kDa



    The lower molecular weight band may represent one of the 3 isoforms of this protein which are known to exist, or a posttranslational modification.

References

This product has been referenced in:

  • Yamamura H  et al. TMEM16A and TMEM16B channel proteins generate Ca2+-activated Cl- current and regulate melatonin secretion in rat pineal glands. J Biol Chem 293:995-1006 (2018). Read more (PubMed: 29187602) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Brain)
Specification
Brain

Abcam user community

Verified customer

Submitted Jan 13 2011

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