Product nameAnti-TMEM173 antibody [EPR13130] (HRP)
See all TMEM173 primary antibodies
DescriptionRabbit monoclonal [EPR13130] to TMEM173 (HRP)
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment within Human TMEM173 aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: Q86WV6
- WB: THP-1 whole cell lysate. IHC-P: normal human spleen tissue sections
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% Proclin
Constituents: PBS, 1% BSA, 30% Glycerol
Concentration information loading...
PurityProtein A purified
- Anti-TMEM173 antibody [EPR13130] (ab181125)
- Anti-TMEM173 antibody [EPR13130] (Alexa Fluor® 488) (ab198950)
- Anti-TMEM173 antibody [EPR13130] (Alexa Fluor® 647) (ab198952)
- Anti-TMEM173 antibody [EPR13130] (Alexa Fluor® 594) (ab207288)
- Anti-TMEM173 antibody [EPR13130] (Phycoerythrin) (ab208874)
- Anti-TMEM173 antibody [EPR13130] - BSA and Azide free (ab227128)
Our Abpromise guarantee covers the use of ab198951 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).|
|IHC-P||1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionFacilitator of innate immune signaling that promotes the production of type I interferon (IFN-alpha and IFN-beta). Innate immune response is triggered in response to non-CpG double-stranded DNA from viruses and bacteria delivered to the cytoplasm. Able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon and exert a potent anti-viral state following expression. May be involved in translocon function, the translocon possibly being able to influence the induction of type I interferons. May be involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II). Mediates death signaling via activation of the extracellular signal-regulated kinase (ERK) pathway.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the TMEM173 family.
modificationsPhosphorylated on tyrosine residues upon MHC-II aggregation (By similarity). Phosphorylated on Ser-358 by TBK1, leading to activation and production of IFN-beta.
Ubiquitinated. 'Lys-63'-linked ubiquitination mediated by TRIM56 at Lys-150 promotes homodimerization and recruitment of the antiviral kinase TBK1 and subsequent production of IFN-beta. 'Lys-48'-linked polyubiquitination at Lys-150 occurring after viral infection is mediated by RNF5 and leads to proteasomal degradation.
Cellular localizationEndoplasmic reticulum membrane. Mitochondrion outer membrane. Cell membrane. Cytoplasm > perinuclear region. In response to double-stranded DNA stimulation, relocalizes to perinuclear region, where the kinase TBK1 is recruited.
- Information by UniProt
- endoplasmic reticulum IFN stimulator antibody
- Endoplasmic reticulum interferon stimulator antibody
- ERIS antibody
IHC image of TMEM173 staining in a section of formalin-fixed paraffin-embedded normal human spleen*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab198951, 1/500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Anti-TMEM173 antibody [EPR13130] (HRP) (ab198951) at 1/5000 dilution + THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time: 2 minutes
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab198951 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab198951 has not yet been referenced specifically in any publications.