Recombinant
RabMAb

Recombinant Anti-TMEM173 antibody [EPR13130] (Phycoerythrin) (ab208874)

Overview

  • Product name

    Anti-TMEM173 antibody [EPR13130] (Phycoerythrin)
    See all TMEM173 primary antibodies
  • Description

    Rabbit monoclonal [EPR13130] to TMEM173 (Phycoerythrin)
  • Host species

    Rabbit
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity


    Predicted to work with: Human
  • Immunogen

    Recombinant fragment within Human TMEM173 aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: Q86WV6

  • Positive control

    • Flow Cyt: HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab208874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500.

The cellular localisation of this product has been verified in ICC/IF

Target

  • Function

    Facilitator of innate immune signaling that promotes the production of type I interferon (IFN-alpha and IFN-beta). Innate immune response is triggered in response to non-CpG double-stranded DNA from viruses and bacteria delivered to the cytoplasm. Able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon and exert a potent anti-viral state following expression. May be involved in translocon function, the translocon possibly being able to influence the induction of type I interferons. May be involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II). Mediates death signaling via activation of the extracellular signal-regulated kinase (ERK) pathway.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the TMEM173 family.
  • Post-translational
    modifications

    Phosphorylated on tyrosine residues upon MHC-II aggregation (By similarity). Phosphorylated on Ser-358 by TBK1, leading to activation and production of IFN-beta.
    Ubiquitinated. 'Lys-63'-linked ubiquitination mediated by TRIM56 at Lys-150 promotes homodimerization and recruitment of the antiviral kinase TBK1 and subsequent production of IFN-beta. 'Lys-48'-linked polyubiquitination at Lys-150 occurring after viral infection is mediated by RNF5 and leads to proteasomal degradation.
  • Cellular localization

    Endoplasmic reticulum membrane. Mitochondrion outer membrane. Cell membrane. Cytoplasm > perinuclear region. In response to double-stranded DNA stimulation, relocalizes to perinuclear region, where the kinase TBK1 is recruited.
  • Information by UniProt
  • Database links

  • Alternative names

    • endoplasmic reticulum IFN stimulator antibody
    • Endoplasmic reticulum interferon stimulator antibody
    • ERIS antibody
    • FLJ38577 antibody
    • hMITA antibody
    • hSTING antibody
    • Mediator of IRF3 activation antibody
    • MITA antibody
    • Mitochondrial mediator of IRF3 activation antibody
    • MPYS antibody
    • N terminal methionine proline tyrosine serine plasma membrane tetraspanner antibody
    • NET23 antibody
    • Stimulator of interferon genes antibody
    • Stimulator of interferon genes protein antibody
    • STING antibody
    • TM173_HUMAN antibody
    • Tmem173 antibody
    • Transmembrane protein 173 antibody
    see all

Images

  • Overlay histogram showing HeLa cells stained with ab208874 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab208874, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

    This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

References

ab208874 has not yet been referenced specifically in any publications.

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