Recombinant
RabMAb

Recombinant Anti-TMEM173 antibody [SP338] - BSA and Azide free (ab238795)

Overview

  • Product name

    Anti-TMEM173 antibody [SP338] - BSA and Azide free
    See all TMEM173 primary antibodies
  • Description

    Rabbit monoclonal [SP338] to TMEM173 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human TMEM173 aa 350 to the C-terminus. The exact sequence is proprietary.
    Database link: Q86WV6

  • Positive control

    • IHC-P: Human tonsil, Human colon, and Rat spleen tissues.
  • General notes

    Ab238795 is the carrier-free version of ab227704. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab238795 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Applications

Our Abpromise guarantee covers the use of ab238795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.
IHC-P Use at an assay dependent concentration.

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Flow Cyt Use at an assay dependent concentration.

Target

  • Function

    Facilitator of innate immune signaling that promotes the production of type I interferon (IFN-alpha and IFN-beta). Innate immune response is triggered in response to non-CpG double-stranded DNA from viruses and bacteria delivered to the cytoplasm. Able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon and exert a potent anti-viral state following expression. May be involved in translocon function, the translocon possibly being able to influence the induction of type I interferons. May be involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II). Mediates death signaling via activation of the extracellular signal-regulated kinase (ERK) pathway.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the TMEM173 family.
  • Post-translational
    modifications

    Phosphorylated on tyrosine residues upon MHC-II aggregation (By similarity). Phosphorylated on Ser-358 by TBK1, leading to activation and production of IFN-beta.
    Ubiquitinated. 'Lys-63'-linked ubiquitination mediated by TRIM56 at Lys-150 promotes homodimerization and recruitment of the antiviral kinase TBK1 and subsequent production of IFN-beta. 'Lys-48'-linked polyubiquitination at Lys-150 occurring after viral infection is mediated by RNF5 and leads to proteasomal degradation.
  • Cellular localization

    Endoplasmic reticulum membrane. Mitochondrion outer membrane. Cell membrane. Cytoplasm > perinuclear region. In response to double-stranded DNA stimulation, relocalizes to perinuclear region, where the kinase TBK1 is recruited.
  • Information by UniProt
  • Database links

  • Alternative names

    • endoplasmic reticulum IFN stimulator antibody
    • Endoplasmic reticulum interferon stimulator antibody
    • ERIS antibody
    • FLJ38577 antibody
    • hMITA antibody
    • hSTING antibody
    • Mediator of IRF3 activation antibody
    • MITA antibody
    • Mitochondrial mediator of IRF3 activation antibody
    • MPYS antibody
    • N terminal methionine proline tyrosine serine plasma membrane tetraspanner antibody
    • NET23 antibody
    • Stimulator of interferon genes antibody
    • Stimulator of interferon genes protein antibody
    • STING antibody
    • TM173_HUMAN antibody
    • Tmem173 antibody
    • Transmembrane protein 173 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling TMEM173 with ab227704 at 1/100 dilution (1.05 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the rat spleen, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab227704 for 10 mins at room temperature. This image was generated using ab227704, the same clone, but with a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling TMEM173 with ab227704 at 1/100 dilution (1.05 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human colon, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab227704 for 10 mins at room temperature. This image was generated using ab227704, the same clone, but with a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling TMEM173 with ab227704 at 1/100 dilution (1.05 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab227704 for 10 mins at room temperature. This image was generated using ab227704, the same clone, but with a different buffer formulation.

  • Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227704).

  • Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227704).

  • Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227704).

  • Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227704).

  • Formalin-fixed, paraffin-embedded human lung tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227704).

  • Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227704).

  • Formalin-fixed, paraffin-embedded human lymph node tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227704).

  • Formalin-fixed, paraffin-embedded human spleen tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227704).

  • Formalin-fixed, paraffin-embedded human thymus tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227704).

  • Flow cytometric analysis of THP-1 (human monocytic leukemia cell line) cell line labeling TMEM173 with ab227704 at 1/400 dilution (green) compared with a rabbit IgG negative control (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227704).

  • Formalin-fixed, paraffin-embedded human colon tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227704).

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for TMEM173 using ab227704 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227704).

References

ab238795 has not yet been referenced specifically in any publications.

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