Recombinant
RabMAb

Recombinant Anti-TMEM173 antibody [SP339] (ab227705)

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Overview

  • Product name

    Anti-TMEM173 antibody [SP339]
    See all TMEM173 primary antibodies

  • Description

    Rabbit monoclonal [SP339] to TMEM173

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Pig

  • Immunogen

    Synthetic peptide within Human TMEM173 aa 150-350 (C terminal). The exact sequence is proprietary.
    Database link: Q86WV6

  • Positive control

    • WB: THP-1 cell lysate. IHC-P: Human tonsil, colon, colon carcinoma, thymus, lymph node, lung, HK lymphoma, lung adenocarcinoma, pancreatic adenocarcinoma, lung squamous cell carcinoma and skin squamous cell carcinoma tissues. FC: THP-1 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab227705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Primary incubation for 10 minutes at room temperature.
WB 1/400. Predicted molecular weight: 42 kDa.

Primary incubation for 1 hour at room temperature.
Flow Cyt 1/20 - 1/400.

Primary incubation for 30 minutes at 4°C.

Target

  • Function

    Facilitator of innate immune signaling that promotes the production of type I interferon (IFN-alpha and IFN-beta). Innate immune response is triggered in response to non-CpG double-stranded DNA from viruses and bacteria delivered to the cytoplasm. Able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon and exert a potent anti-viral state following expression. May be involved in translocon function, the translocon possibly being able to influence the induction of type I interferons. May be involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II). Mediates death signaling via activation of the extracellular signal-regulated kinase (ERK) pathway.

  • Tissue specificity

    Ubiquitously expressed.

  • Sequence similarities

    Belongs to the TMEM173 family.

  • Post-translational
    modifications

    Phosphorylated on tyrosine residues upon MHC-II aggregation (By similarity). Phosphorylated on Ser-358 by TBK1, leading to activation and production of IFN-beta.
    Ubiquitinated. 'Lys-63'-linked ubiquitination mediated by TRIM56 at Lys-150 promotes homodimerization and recruitment of the antiviral kinase TBK1 and subsequent production of IFN-beta. 'Lys-48'-linked polyubiquitination at Lys-150 occurring after viral infection is mediated by RNF5 and leads to proteasomal degradation.

  • Cellular localization

    Endoplasmic reticulum membrane. Mitochondrion outer membrane. Cell membrane. Cytoplasm > perinuclear region. In response to double-stranded DNA stimulation, relocalizes to perinuclear region, where the kinase TBK1 is recruited.
  • Information by UniProt

  • Database links

    • Alternative names

      • endoplasmic reticulum IFN stimulator antibody
      • Endoplasmic reticulum interferon stimulator antibody
      • ERIS antibody
      • FLJ38577 antibody
      • hMITA antibody
      • hSTING antibody
      • Mediator of IRF3 activation antibody
      • MITA antibody
      • Mitochondrial mediator of IRF3 activation antibody
      • MPYS antibody
      • N terminal methionine proline tyrosine serine plasma membrane tetraspanner antibody
      • NET23 antibody
      • Stimulator of interferon genes antibody
      • Stimulator of interferon genes protein antibody
      • STING antibody
      • TM173_HUMAN antibody
      • Tmem173 antibody
      • Transmembrane protein 173 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM173 antibody [SP339] (ab227705)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM173 antibody [SP339] (ab227705)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling TMEM173 with ab227705 at 1/100 dilution (2.5 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human colon carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
      The section was incubated with ab227705 for 10 mins at room temperature.

    • Western blot - Anti-TMEM173 antibody [SP339] (ab227705)
      Western blot - Anti-TMEM173 antibody [SP339] (ab227705)
      Anti-TMEM173 antibody [SP339] (ab227705) at 1/400 dilution + THP-1 (human monocytic leukemia cell line) cell lysate

      Predicted band size: 42 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM173 antibody [SP339] (ab227705)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM173 antibody [SP339] (ab227705)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling TMEM173 with ab227705 at 1/100 dilution (2.5 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
      The section was incubated with ab227705 for 10 mins at room temperature.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM173 antibody [SP339] (ab227705)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM173 antibody [SP339] (ab227705)

      Formalin-fixed, paraffin-embedded human thymus tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

    • Flow Cytometry - Anti-TMEM173 antibody [SP339] (ab227705)
      Flow Cytometry - Anti-TMEM173 antibody [SP339] (ab227705)

      Flow cytometry analysis of THP-1 (human acute monocytic leukemia) labeling TMEM173 with purified ab227705 at 1/20 dilution (12.5 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlableled control - Unlabelled cells (blue).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM173 antibody [SP339] (ab227705)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM173 antibody [SP339] (ab227705)

      Formalin-fixed, paraffin-embedded human tonsil tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

    • Flow Cytometry - Anti-TMEM173 antibody [SP339] (ab227705)
      Flow Cytometry - Anti-TMEM173 antibody [SP339] (ab227705)

      Flow cytometric analysis of THP-1 (human monocytic leukemia cell line) cell line labeling TMEM173 with ab227705 at 1/400 dilution (green) compared with a Rabbit IgG negative control (blue).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM173 antibody [SP339] (ab227705)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM173 antibody [SP339] (ab227705)

      Formalin-fixed, paraffin-embedded human colon tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM173 antibody [SP339] (ab227705)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM173 antibody [SP339] (ab227705)

      Formalin-fixed, paraffin-embedded human lymph node tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM173 antibody [SP339] (ab227705)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM173 antibody [SP339] (ab227705)

      Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM173 antibody [SP339] (ab227705)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM173 antibody [SP339] (ab227705)

      Formalin-fixed, paraffin-embedded human lung tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM173 antibody [SP339] (ab227705)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM173 antibody [SP339] (ab227705)

      Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM173 antibody [SP339] (ab227705)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM173 antibody [SP339] (ab227705)

      Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM173 antibody [SP339] (ab227705)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM173 antibody [SP339] (ab227705)

      Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM173 antibody [SP339] (ab227705)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM173 antibody [SP339] (ab227705)

      Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM173 antibody [SP339] (ab227705)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM173 antibody [SP339] (ab227705)

      Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for TMEM173 using ab227705 at 1/100 dilution in immunohistochemical analysis.

    References

    ab227705 has not yet been referenced specifically in any publications.

    Publishing research using ab227705? Please let us know so that we can cite the reference in this datasheet.

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