Overview

  • Product name

    Anti-TMEM180 antibody
  • Description

    Rabbit polyclonal to TMEM180
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 361-411 (CLFIASNRVF TEGTCKLLTL VVTDLVDEDL VLNHRKQAAS ALLFGMVALV T) of Human TMEM180 (NP_079065).

  • Positive control

    • THP-1 cell lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab108080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 57 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Anti-TMEM180 antibody (ab108080) at 1 µg/ml + THP-1 cell lysate at 10 µg

    Predicted band size: 57 kDa



    Gel concentration: 12%

     

    Secondary antibody - anti-rabbit HRP (ab6721)

References

ab108080 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (Skin Fibroblasts)
Loading amount
40 µg
Specification
Skin Fibroblasts
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 07 2013

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Skin fibroblasts)
Specification
Skin fibroblasts
Fixative
Methanol
Permeabilization
Yes - 0.1% triton
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 06 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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