Western blot abreview for Anti-TMEM180 antibody

Average
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Skin Fibroblasts)
Loading amount
40 µg
Specification
Skin Fibroblasts
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/500
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST+5%milk

Secondary antibody

Dilution
1/10000

Detection

Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: ~55 kDa Non-specific: >70, >170 kDa

Additional data

Additional Notes
Two independent samples from different sources of skin fibroblast cells show similar pattern.
We suspect that the weak band <55 is the right one.

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Submitted May 07 2013

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