Anti-TMEM19 antibody (ab236852)
Key features and details
- Rabbit polyclonal to TMEM19
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TMEM19 antibody -
Description
Rabbit polyclonal to TMEM19 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Orangutan -
Immunogen
Recombinant fragment corresponding to Human TMEM19 aa 100-250.
Database link: Q96HH6 -
Positive control
- WB: MCF7, U-251 MG and A549 whole cell lysates; Mouse stomach lysate. IHC-P: Human lung, gastric cancer and breast cancer tissues. ICC/IF: A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236852 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 36 kDa.
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IHC-P |
1/20 - 1/200.
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ICC/IF |
1/50 - 1/200.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 36 kDa. |
IHC-P
1/20 - 1/200. |
ICC/IF
1/50 - 1/200. |
Target
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Sequence similarities
Belongs to the TMEM19 family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 55266 Human
- Entrez Gene: 67226 Mouse
- Entrez Gene: 100171546 Orangutan
- Entrez Gene: 299800 Rat
- SwissProt: Q96HH6 Human
- SwissProt: Q91W52 Mouse
- SwissProt: Q5RF73 Orangutan
- SwissProt: Q6P726 Rat
see all -
Alternative names
- tmem19 antibody
- TMM19_HUMAN antibody
- Transmembrane protein 19 antibody
Images
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All lanes : Anti-TMEM19 antibody (ab236852) at 1/500 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) whole cell lysate
Lane 3 : Mouse stomach lysate
Lane 4 : A549 (human lung carcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 36 kDa -
A549 (human lung carcinoma cell line) cells stained for TMEM19 (green) using ab236852 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
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Paraffin-embedded human lung tissue stained for TMEM19 using ab236852 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human breast cancer tissue stained for TMEM19 using ab236852 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human gastric cancer tissue stained for TMEM19 using ab236852 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab236852 has not yet been referenced specifically in any publications.