Key features and details
- Rabbit polyclonal to TMEM19
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-TMEM19 antibody
See all TMEM19 primary antibodies
DescriptionRabbit polyclonal to TMEM19
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Orangutan
- WB: MCF7, U-251 MG and A549 whole cell lysates; Mouse stomach lysate. IHC-P: Human lung, gastric cancer and breast cancer tissues. ICC/IF: A549 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol, PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab236852 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 36 kDa.|
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
Sequence similaritiesBelongs to the TMEM19 family.
- Information by UniProt
- tmem19 antibody
- TMM19_HUMAN antibody
- Transmembrane protein 19 antibody
All lanes : Anti-TMEM19 antibody (ab236852) at 1/500 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) whole cell lysate
Lane 3 : Mouse stomach lysate
Lane 4 : A549 (human lung carcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 36 kDa
A549 (human lung carcinoma cell line) cells stained for TMEM19 (green) using ab236852 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
Paraffin-embedded human lung tissue stained for TMEM19 using ab236852 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human breast cancer tissue stained for TMEM19 using ab236852 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human gastric cancer tissue stained for TMEM19 using ab236852 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236852 has not yet been referenced specifically in any publications.