Recombinant Anti-TMEM192 antibody [EPR14330-67] (ab186737)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14330-67] to TMEM192
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TMEM192 antibody [EPR14330-67]
See all TMEM192 primary antibodies -
Description
Rabbit monoclonal [EPR14330-67] to TMEM192 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
This product was produced with the following immunogens:
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers. -
Positive control
- U-87 MG, Human fetal liver and Human fetal lung lysates; U-87 MG cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14330-67 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186737 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
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Flow Cyt (Intra) |
1/180.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa). |
Flow Cyt (Intra)
1/180. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Tissue specificity
Strongly expressed in kidney, liver, lung and pancreas. -
Sequence similarities
Belongs to the TMEM192 family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Not N-glycosylated. -
Cellular localization
Lysosome membrane. Late endosome. - Information by UniProt
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Database links
- Entrez Gene: 201931 Human
- SwissProt: Q8IY95 Human
- Unigene: 704511 Human
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Alternative names
- FLJ38482 antibody
- TM192_HUMAN antibody
- TMEM192 antibody
- Transmembrane protein 192 antibody
Images
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All lanes : Anti-TMEM192 antibody [EPR14330-67] (ab186737) at 1/10000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : Human fetal liver lysate
Lane 3 : Human fetal lung lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa -
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixedU-87 MG cells labeling TMEM192 with ab186737 at 1/180 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed bygoat anti-rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab186737 has been referenced in 1 publication.
- Sharma C et al. Antioxidant functions of DHHC3 suppress anti-cancer drug activities. Cell Mol Life Sci 78:2341-2353 (2021). PubMed: 32986127