Key features and details
- Rabbit polyclonal to TMEM2
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TMEM2 antibody
See all TMEM2 primary antibodies
DescriptionRabbit polyclonal to TMEM2
Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human TMEM2 aa 734-866.
Database link: Q9UHN6
- IHC-P: Human brain tissue. ICC/IF: A549 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%.
Our Abpromise guarantee covers the use of ab238740 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/200 - 1/500.|
FunctionMay be required for the heart morphogenesis.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the TMEM2 family.
Contains 1 G8 domain.
Contains 3 PbH1 repeats.
- Information by UniProt
- KIAA1412 antibody
- TMEM 2 antibody
- tmem2 antibody
Paraffin-embedded human brain tissue stained for TMEM2 using ab238740 at 1/300 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
A549 (Human lung carcinoma cell line) cells stained for TMEM2 (Green) using ab238740 at 1/100 dilution in ICC/IF, followed by an Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C. The nuclear counterstain is DAPI.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab238740 has not yet been referenced specifically in any publications.