Anti-TMEM208 antibody (ab130459)

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Overview

  • Product name

    Anti-TMEM208 antibody
    See all TMEM208 primary antibodies

  • Description

    Rabbit polyclonal to TMEM208

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment: PWFTADSGTPAPEHNEKRQRRQERRQMKRL, corresponding to amino acids 144-173 of Human TMEM208 Isoform 1 (Q9BTX3).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human rectum tissue.

  • General notes

    The antibody solution should be gently mixed before use. ab139459 is a mono-specific antibody. Working dilution samples should be discarded if not used within 12 hours.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab130459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Western blot - Anti-TMEM208 antibody (ab130459)
    Western blot - Anti-TMEM208 antibody (ab130459)
    Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
    Lane 2: Negative control (vector only transfected HEK293T lysate)
    Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cell.
  • Immunocytochemistry/ Immunofluorescence - Anti-TMEM208 antibody (ab130459)
    Immunocytochemistry/ Immunofluorescence - Anti-TMEM208 antibody (ab130459)
    Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm. Recommended concentration of ab130459 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM208 antibody (ab130459)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM208 antibody (ab130459)
    ab130459, at 1/20 dilution, staining TMEM208 in paraffin-embedded Human rectum tissue by Immunohistochemistry

References

ab130459 has not yet been referenced specifically in any publications.

Publishing research using ab130459? Please let us know so that we can cite the reference in this datasheet.

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