Key features and details
- Rabbit polyclonal to TMEM25
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TMEM25 antibody
DescriptionRabbit polyclonal to TMEM25
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
antigen, corresponding to amino acids 262-364 of Human TMEM25 (Uniprot: Q86YD3)
- Human Liver tissue and Liver lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121362 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|IHC-P||1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/250 - 1/500.|
Sequence similaritiesContains 1 Ig-like (immunoglobulin-like) domain.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- 0610039J01Rik antibody
- FLJ14399 antibody
- Tmem25 antibody
All lanes : Anti-TMEM25 antibody (ab121362) at 1/250 dilution
Lane 1 : RT-4 lysate
Lane 2 : U-251 MG lysate
Lane 3 : Human Plasma lysate
Lane 4 : Liver lysate
Lane 5 : Tonsil lysate
ab121362, at 1/10 dilution, staining Paraffin-embedded Human Liver tissue by Immunohistochemistry.
Immunofluorescent staining of human cell line A-431 shows positivity in vesicles.
ab121362 has not yet been referenced specifically in any publications.