Overview

  • Product name

    Anti-TMEM256 antibody [EPR12842]
    See all TMEM256 primary antibodies
  • Description

    Rabbit monoclonal [EPR12842] to TMEM256
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human TMEM256 aa 1-100. The exact sequence is proprietary.
    Database link: Q8N2U0

  • Positive control

    • Human fetal liver, fetal kidney, A549, Y79 and LnCaP lysates; A549 cells.
  • General notes

     

     

     This product was previously labelled as C17orf61

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR12842
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
IP 1/30 - 1/50.
Flow Cyt 1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

Images

  • All lanes : Anti-TMEM256 antibody [EPR12842] (ab181239) at 1/10000 dilution

    Lane 1 : A549 cell lysate
    Lane 2 : Y79 cell lysate
    Lane 3 : LnCaP cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 12 kDa

  • Flow cytometric analysis of A549 cells (paraformaldehyde-fixed, 2%) labeling TMEM256 with ab181239 at 1/200 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Western blot analysis of immunoprecipitation pellet from A549 cell lysate immunoprecipitated using ab181239 at 1/50 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

  • All lanes : Anti-TMEM256 antibody [EPR12842] (ab181239) at 1/2000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution (Goat Anti-Rabbit IgG HRP)

    Predicted band size: 12 kDa

References

ab181239 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181239.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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