Overview

  • Product name

  • Description

    Rabbit polyclonal to Tmem27
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    Recombinant full length protein corresponding to Mouse Tmem27 aa 12-222. Two N-terminal tags, Hist -tag and GST-tag. Expressed in E.coli.
    Sequence:

    IHAELCHPDAENAFKVRLSIRAALGDKAYVWDTDQEYLFRAMVAFSMRKV PNREATEISHVLLCNITQRVSFWFVVTDPSNNYTLPAAEVQSAIRKNRNR INSAFFLDDHTLEFLKIPSTLAPPMEPSVPVWIIVFGVIFCIVTVAIALL VLSGIRQRRRNNKGPPGVEDAEDKCENIITIENGIPCDPLDMKGGHINDG FLTEDERLTPL


    Database link: Q9ESG4

  • Positive control

    • IHC-P: Mouse kidney, stomach and pancreas tissue. WB: Recombinant mouse Tmem27 protein; Mouse pancreas lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab233421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 25 kDa.
IHC-P Use a concentration of 5 - 20 µg/ml.

Target

  • Function

    Regulator of SNARE complex function. Stimulator of beta cell replication.
  • Tissue specificity

    Kidney; collecting ducts. Pancreas; beta cells of islets.
  • Sequence similarities

    Belongs to the TMEM27 family.
  • Post-translational
    modifications

    Glycosylated.
    The extracellular domain is cleaved and released from the cell membrane of pancreatic beta cells.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • UNQ679 antibody
    • Collectrin antibody
    • kidney-specific membrane protein antibody
    • NX-17 antibody
    • NX17 antibody
    • PRO1312 antibody
    • Tmem27 antibody
    • TMM27_HUMAN antibody
    • Transmembrane protein 27 antibody
    • UNQ679/PRO1312 antibody
    see all

Images

  • Anti-Tmem27 antibody (ab233421) at 30000 µg/ml + Mouse pancreas tissue lysate.

    Secondary
    HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

    Predicted band size: 25 kDa

  • Anti-Tmem27 antibody (ab233421) at 3 mg/ml + Recombinant mouse Tmem27 protein.

    Secondary
    HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

    Predicted band size: 25 kDa

  • Formalin-fixed, paraffin-embedded mouse pancreas tissue stained for Tmem27 using ab233421 at 20 µg/mL in immunohistochemical analysis. DAB staining.

  • Formalin-fixed, paraffin-embedded mouse stomach tissue stained for Tmem27 using ab233421 at 20 µg/mL in immunohistochemical analysis. DAB staining.

  • Formalin-fixed, paraffin-embedded mouse kidney tissue stained for Tmem27 using ab233421 at 20 µg/mL in immunohistochemical analysis. DAB staining.

References

ab233421 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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