Anti-Tmem27 antibody (ab233531)
Key features and details
- Rabbit polyclonal to Tmem27
- Suitable for: WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-Tmem27 antibody
See all Tmem27 primary antibodies -
Description
Rabbit polyclonal to Tmem27 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Rat Tmem27 aa 1-150. Two N-terminal tags. Expressed in E.coli.
Database link: Q9ESG3 -
Positive control
- WB: Recombinant rat Tmem27protein; Rat serum. IHC-P: Rat kidney tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233531 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 25 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 25 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Regulator of SNARE complex function. Stimulator of beta cell replication. -
Tissue specificity
Kidney; collecting ducts. Pancreas; beta cells of islets. -
Sequence similarities
Belongs to the TMEM27 family. -
Post-translational
modificationsGlycosylated.
The extracellular domain is cleaved and released from the cell membrane of pancreatic beta cells. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 57394 Mouse
- Entrez Gene: 57395 Rat
- SwissProt: Q9ESG4 Mouse
- SwissProt: Q9ESG3 Rat
- Unigene: 143766 Mouse
- Unigene: 32298 Rat
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Alternative names
- UNQ679 antibody
- Collectrin antibody
- kidney-specific membrane protein antibody
see all
Images
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Formalin-fixed, paraffin-embedded rat kidney tissue stained for Tmem27 using ab233531 at 20μg/ml in immunohistochemical analysis. DAB staining.
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Anti-Tmem27 antibody (ab233531) at 2 µg/ml + Recombinant rat Tmem27 protein
Developed using the ECL technique.
Predicted band size: 25 kDa -
Anti-Tmem27 antibody (ab233531) at 2 µg/ml + Rat serum
Developed using the ECL technique.
Predicted band size: 25 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab233531 has not yet been referenced specifically in any publications.