Anti-Tmem27 antibody (ab233531)

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Overview

  • Product name

    Anti-Tmem27 antibody
    See all Tmem27 primary antibodies

  • Description

    Rabbit polyclonal to Tmem27

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse

  • Immunogen

    Recombinant fragment (His-T7-tag) corresponding to Rat Tmem27 aa 15-141. Two N-terminal tags. Expressed in E.coli.
    Sequence:

    ELCRPDAENAFKVRLSIKAALGDKAYVWDTDEEYLFRAMVAFSMRKVPNR EGTEISHVLLCNVTQRVSFWFVVTDPLKNHTLPAAEVQSAIRMNRNRINS AFFLDDHTLEFLKIPSTLAPPMDPSVP


    Database link: Q9ESG3

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Recombinant rat Tmem27protein; Rat serum. IHC-P: Rat kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab233531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 25 kDa.
IHC-P Use a concentration of 5 - 20 µg/ml.

Target

  • Function

    Regulator of SNARE complex function. Stimulator of beta cell replication.

  • Tissue specificity

    Kidney; collecting ducts. Pancreas; beta cells of islets.

  • Sequence similarities

    Belongs to the TMEM27 family.

  • Post-translational
    modifications

    Glycosylated.
    The extracellular domain is cleaved and released from the cell membrane of pancreatic beta cells.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • UNQ679 antibody
      • Collectrin antibody
      • kidney-specific membrane protein antibody
      • NX-17 antibody
      • NX17 antibody
      • PRO1312 antibody
      • Tmem27 antibody
      • TMM27_HUMAN antibody
      • Transmembrane protein 27 antibody
      • UNQ679/PRO1312 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tmem27 antibody (ab233531)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tmem27 antibody (ab233531)

      Formalin-fixed, paraffin-embedded rat kidney tissue stained for Tmem27 using ab233531 at 20μg/ml in immunohistochemical analysis. DAB staining.

    • Western blot - Anti-Tmem27 antibody (ab233531)
      Western blot - Anti-Tmem27 antibody (ab233531)
      Anti-Tmem27 antibody (ab233531) at 2 µg/ml + Recombinant rat Tmem27 protein

      Developed using the ECL technique.

      Predicted band size: 25 kDa

    • Western blot - Anti-Tmem27 antibody (ab233531)
      Western blot - Anti-Tmem27 antibody (ab233531)
      Anti-Tmem27 antibody (ab233531) at 2 µg/ml + Rat serum

      Developed using the ECL technique.

      Predicted band size: 25 kDa

    References

    ab233531 has not yet been referenced specifically in any publications.

    Publishing research using ab233531? Please let us know so that we can cite the reference in this datasheet.

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