Recombinant Anti-TMEM30B antibody [EPR14409] - BSA and Azide free (ab236133)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14409] to TMEM30B - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-TMEM30B antibody [EPR14409] - BSA and Azide free
See all TMEM30B primary antibodies -
Description
Rabbit monoclonal [EPR14409] to TMEM30B - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MCF7 and BxPC-3 cell lysate.
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General notes
ab236133 is the carrier-free version of ab185944.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14409 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236133 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). |
Target
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Sequence similarities
Belongs to the CDC50/LEM3 family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 161291 Human
- Entrez Gene: 238257 Mouse
- Entrez Gene: 314224 Rat
- Omim: 611029 Human
- SwissProt: Q3MIR4 Human
- SwissProt: Q8BHG3 Mouse
- Unigene: 146180 Human
- Unigene: 251196 Mouse
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Alternative names
- 9130011B11Rik antibody
- CC50B_HUMAN antibody
- CDC50, S. cerevisiae, homolog of, B antibody
see all
Images
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All lanes : Anti-TMEM30B antibody [EPR14409] (ab185944) at 1/10000 dilution
Lane 1 : BxPC-3 cell lysate
Lane 2 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 39 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185944).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab236133 has not yet been referenced specifically in any publications.