Overview

  • Product name

  • Description

    Rabbit polyclonal to TMEM33
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TMEM33 aa 197-247. The exact sequence is proprietary.
    Database link: P57088

  • Positive control

    • WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HEK-293T whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibody was affinity purified using an epitope specific to TMEM33 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab242108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 28 kDa.
IP Use at an assay dependent concentration.

20 µl/mg lysate

Target

  • Sequence similarities

    Belongs to the PER33/POM33 family.
  • Cellular localization

    Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1600019D15Rik antibody
    • FLJ10525 antibody
    • OTTHUMP00000125225 antibody
    • OTTHUMP00000218396 antibody
    • OTTHUMP00000218397 antibody
    • Protein DB83 antibody
    • SHINC3 antibody
    • tmem33 antibody
    • TMM33_HUMAN antibody
    • Transmembrane protein 33 antibody
    see all

Images

  • All lanes : Anti-TMEM33 antibody (ab242108) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 28 kDa


    Exposure time: 10 seconds


    Lysates prepared in NETN buffer.

  • TMEM33 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab242108 at 20 µl/reaction. Western blot was performed from the immunoprecipitate using ab242108 at 1/100 dilution.

    Lane 1: ab242108 IP in HEK-293T whole cell lysate.
    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with exposure time of 30 seconds.

    Lysates prepared in NETN buffer.

References

ab242108 has not yet been referenced specifically in any publications.

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