Key features and details
- Rabbit polyclonal to TMEM43
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-TMEM43 antibody
See all TMEM43 primary antibodies
DescriptionRabbit polyclonal to TMEM43
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Orangutan
- WB: A549 whole cell lysate; Mouse brain and skeletal muscle tissue lysates. IHC-P: Human melanoma cancer tissue.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab224629 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).|
|IHC-P||1/20 - 1/200.|
FunctionMay have an important role in maintaining nuclear envelope structure by organizing protein complexes at the inner nuclear membrane. Required for retaining emerin at the inner nuclear membrane.
Tissue specificityHighest expression in placenta. Also found at lower levels in heart, ovary, spleen, small intestine, thymus, prostate and testis.
Involvement in diseaseDefects in TMEM43 are the cause of familial arrhythmogenic right ventricular dysplasia type 5 (ARVD5) [MIM:604400]; also known as arrhythmogenic right ventricular cardiomyopathy (ARVC5). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall.
Sequence similaritiesBelongs to the TMEM43 family.
Cellular localizationEndoplasmic reticulum. Nucleus inner membrane. Retained in the inner nuclear membrane through interaction with EMD and A-and B-lamins. The N- and C-termini are orientated towards the nucleoplasm. The majority of the hydrophilic domain resides in the endoplasmic reticulum lumen.
- Information by UniProt
- ARVC5 antibody
- ARVD5 antibody
- EDMD7 antibody
All lanes : Anti-TMEM43 antibody (ab224629) at 1/500 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Mouse skeletal muscle tissue lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
Paraffin-embedded human melanoma cancer tissue stained for TMEM43 using ab224629 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224629 has not yet been referenced specifically in any publications.