Overview

  • Product name

    Anti-TMEM43 antibody [EPR15379]
    See all TMEM43 primary antibodies
  • Description

    Rabbit monoclonal [EPR15379] to TMEM43
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TMEM43 aa 200-300. The exact sequence is proprietary.
    Database link: Q9BTV4

  • Positive control

    • Caco-2 cells; Rat liver tissue; Human transitional cell carcinoma of bladder tissue; Human spleen, placenta and ovary cancer lysates; JAR, MDA-MB-435 and Caco-2 cell lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab190793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function

      May have an important role in maintaining nuclear envelope structure by organizing protein complexes at the inner nuclear membrane. Required for retaining emerin at the inner nuclear membrane.
    • Tissue specificity

      Highest expression in placenta. Also found at lower levels in heart, ovary, spleen, small intestine, thymus, prostate and testis.
    • Involvement in disease

      Defects in TMEM43 are the cause of familial arrhythmogenic right ventricular dysplasia type 5 (ARVD5) [MIM:604400]; also known as arrhythmogenic right ventricular cardiomyopathy (ARVC5). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall.
    • Sequence similarities

      Belongs to the TMEM43 family.
    • Cellular localization

      Endoplasmic reticulum. Nucleus inner membrane. Retained in the inner nuclear membrane through interaction with EMD and A-and B-lamins. The N- and C-termini are orientated towards the nucleoplasm. The majority of the hydrophilic domain resides in the endoplasmic reticulum lumen.
    • Information by UniProt
    • Database links

    • Alternative names

      • ARVC5 antibody
      • ARVD5 antibody
      • EDMD7 antibody
      • LUMA antibody
      • Protein LUMA antibody
      • Tmem43 antibody
      • TMM43_HUMAN antibody
      • Transmembrane protein 43 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder labeling TMEM43 with ab190793 at 1/250 dilution followed by prediluted HRP polymer for Rabbit IgG.  Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling TMEM43 with ab190793 at 1/250 dilution followed by prediluted HRP polymer for Rabbit IgG. Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • All lanes : Anti-TMEM43 antibody [EPR15379] (ab190793) at 1/1000 dilution

      Lane 1 : Human spleen lysate
      Lane 2 : Human placenta lysate
      Lane 3 : Human ovary cancer lysate
      Lane 4 : JAR cell lysate
      Lane 5 : MDA-MB-435 cell lysate
      Lane 6 : Caco-2 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 45 kDa
      Observed band size: 45 kDa

    References

    ab190793 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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