Overview

  • Product name

    Anti-TMEM45B antibody
  • Description

    Rabbit polyclonal to TMEM45B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen sequence:

    EQFVPDGPHLHLYHENHWIKLMNWQ

    , corresponding to amino acids 64-88 of Human TMEM45B.

  • Positive control

    • Human duodenum tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121488 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121488 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121488, at 1/90 dilution, staining TMEM45B in paraffin-embedded Human duodenum tissue by Immunohistochemistry, showing positivity in glandular cells.

References

ab121488 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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