Anti-TMEM49/VMP1 antibody (ab69224)
Key features and details
- Mouse polyclonal to TMEM49/VMP1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TMEM49/VMP1 antibody
See all TMEM49/VMP1 primary antibodies -
Description
Mouse polyclonal to TMEM49/VMP1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human TMEM49/VMP1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- TMEM49/VMP1 transfected 293T lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab69224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). |
Target
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Function
Stress-induced protein that, when overexpressed, promotes formation of intracellular vacuoles followed by cell death. May be involved in the cytoplasmic vacuolization of acinar cells during the early stage of acute pancreatitis. Plays a role in the initial stages of the autophagic process through its interaction with BECN1 (By similarity). Involved in cell-cell adhesion. Plays an essential role in formation of cell junctions. -
Sequence similarities
Belongs to the VMP1 family. -
Cellular localization
Endoplasmic reticulum-Golgi intermediate compartment membrane. Cell membrane. Vacuole membrane. Endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 81671 Human
- Omim: 611753 Human
- SwissProt: Q96GC9 Human
- Unigene: 444569 Human
- Unigene: 708260 Human
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Alternative names
- DKFZP566I133 antibody
- ectopic P-granules autophagy protein 3 homolog antibody
- NF E2 induceble protein 2 antibody
see all
Images
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All lanes : Anti-TMEM49/VMP1 antibody (ab69224) at 1/500 dilution
Lane 1 : TMEM49/VMP1 transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Secondary
Lane 1 : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Lane 2 : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Datasheets and documents
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Datasheet download
References (2)
ab69224 has been referenced in 2 publications.
- Qu A et al. Hypoxia-inducible MiR-210 is an independent prognostic factor and contributes to metastasis in colorectal cancer. PLoS One 9:e90952 (2014). WB ; Human . PubMed: 24632577
- Lo Ré AE et al. Novel AKT1-GLI3-VMP1 Pathway Mediates KRAS Oncogene-induced Autophagy in Cancer Cells. J Biol Chem 287:25325-34 (2012). WB ; Human . PubMed: 22535956