Key features and details
- Rabbit polyclonal to TMEM65
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-TMEM65 antibody
DescriptionRabbit polyclonal to TMEM65
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow
Recombinant full length protein corresponding to Human TMEM65 aa 63-240.
MEALNTAQGARDFIYSLHSTERSCLLKELHRFESIAIAQEKLEAPPPTPG QLRYVFIHNAIPFIGFGFLDNAIMIVAGTHIEMSIGIILGISTMAAAALG NLVSDLAGLGLAGYVEALASRLGLSIPDLTPKQVDMWQTRLSTHLGKAVG VTIGCILGMFPLIFFGGGEEDEKLETKS
Database link: Q6PI78
- WB: Mouse liver, kidney and gonadal tissue lysate. IHC-P: Human placenta and rectum tissue. ICC/IF: HeLa cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab236861 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 25 kDa.|
|ICC/IF||1/50 - 1/200.|
- Information by UniProt
- TMEM65 antibody
- TMM65_HUMAN antibody
- Transmembrane protein 65 antibody
All lanes : Anti-TMEM65 antibody (ab236861) at 1/500 dilution
Lane 1 : Mouse liver lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse gonadal lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 25 kDa
HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling TMEM65 using ab236861 at 1/100 dilution in ICC/IF. Secondary antibody was Alexa Fluor®488-conjugated goat anti-rabbit IgG (H+L).
Paraffin-embedded human placenta tissue stained for TMEM65 using ab236861 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human rectum tissue stained for TMEM65 using ab236861 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236861 has not yet been referenced specifically in any publications.