Anti-TMEM66/SARAF antibody (ab154538)
Key features and details
- Rabbit polyclonal to TMEM66/SARAF
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TMEM66/SARAF antibody -
Description
Rabbit polyclonal to TMEM66/SARAF -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Recombinant fragment corresponding to Human TMEM66/SARAF aa 1-211.
Database link: Q96BY9 -
Positive control
- 293T, Jurkat, Raji whole cell lysate; Human hepatoma tissue; HeLa cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154538 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 37 kDa.
|
IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
ICC/IF |
1/100 - 1/1000.
|
Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 37 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Tissue specificity
Highly expressed in macrophages. -
Sequence similarities
Belongs to the TMEM66 family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 51669 Human
- Entrez Gene: 67887 Mouse
- Omim: 6147768 Human
- SwissProt: Q96BY9 Human
- SwissProt: Q8R3Q0 Mouse
- Unigene: 521487 Human
- Unigene: 290353 Mouse
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Alternative names
- FLJ22274 antibody
- FOAP 7 antibody
- HBV X transactivated gene 3 protein antibody
see all
Images
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Anti-TMEM66/SARAF antibody (ab154538) at 1/1000 dilution + Jurkat whole cell lysate at 30 µg
Predicted band size: 37 kDa -
Immunohistochemical analysis of paraffin-embedded Human hepatoma tissue labeling TMEM66/SARAF with ab154538 at 1/500 dilution.
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Immunofluorescent analysis of methanol-fixed HeLa cells labeling TMEM66/SARAF with ab154538 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab154538 has been referenced in 1 publication.
- Sathish V et al. Cigarette Smoke and Estrogen Signaling in Human Airway Smooth Muscle. Cell Physiol Biochem 36:1101-15 (2015). PubMed: 26113109