Overview

  • Product name

    Anti-TMEM97 antibody
  • Description

    Rabbit polyclonal to TMEM97
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen sequence: FEDFSKASGFKGQRPETLHERLT, corresponding to amino acids 120-142 of Human TMEM97.

  • Positive control

    • Human pancreas tissue; Human cell line U-2 OS.

Properties

Applications

Our Abpromise guarantee covers the use of ab126490 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100.

Target

  • Function

    Plays a role as a regulator of cellular cholesterol homeostasis.
  • Tissue specificity

    Widely expressed in normal tissues. Expressed in pancreatic, renal, breast, colon, ovarian surface epithelial (OSE) cells and meningioma cancers. Expressed in ovarian cancer; expression is reduced relative to OSE cells.
  • Sequence similarities

    Belongs to the TMEM97 family.
  • Cellular localization

    Nucleus membrane. Rough endoplasmic reticulum. Cell membrane. Lysosome. Membrane. Localized in sterol-depleted cells at cell membrane and in lysosomes.
  • Information by UniProt
  • Database links

  • Alternative names

    • MAC30 antibody
    • Protein MAC30 antibody
    • Tmem97 antibody
    • TMM97_HUMAN antibody
    • Transmembrane protein 97 antibody
    see all

Images

  • Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
    Lane 2: Negative control (vector only transfected HEK293T lysate)
    Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
  • ab126490 at 1/50 dilution staining TMEM97 in paraffin-embedded Human pancreas tissue by Immunohistochemistry.

References

ab126490 has not yet been referenced specifically in any publications.

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