Overview

  • Product name

    Anti-TMEPAI antibody
  • Description

    Rabbit polyclonal to TMEPAI
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to an internal region of Human TMEPAI.

  • Positive control

    • HT29 cell extract, Human Prostate, Smooth Muscle tissue, HuvEc cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: (without Mg2+, Ca2+)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab128006 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 32 kDa.
ELISA 1/20000.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.

Target

  • Function

    Involved in down-regulation of the androgen receptor (AR). Enhances ubiquitination and proteasome-mediated degradation of AR, probably by recruiting NEDD4.
  • Tissue specificity

    Highest expression in prostate. Also expressed in ovary.
  • Sequence similarities

    Belongs to the PMEPA1 family.
  • Domain

    The WW-binding motifs mediate interaction with NEDD4.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • PMEPA_HUMAN antibody
    • PMEPA1 antibody
    • Prostate transmembrane protein, androgen induced 1 antibody
    • Solid tumor associated 1 protein antibody
    • Solid tumor-associated 1 protein antibody
    • STAG1 antibody
    • TMEPAI antibody
    • Transmembrane prostate androgen induced protein antibody
    • Transmembrane prostate androgen-induced protein antibody
    • Transmembrane, prostate androgen induced RNA antibody
    see all

Images

  • All lanes : Anti-TMEPAI antibody (ab128006) at 1/100 dilution

    Lane 1 : HT29 cell extract
    Lane 2 : HT29 cell extract with synthesized peptide

    Predicted band size: 32 kDa

  • ab128006, at a concentration of 10 µg/ml, staining TMEPAI in paraffin-embedded Human Prostate, Smooth Muscle tissue by Immunohistochemistry.
  • ab128006, at a dilution of 1/100, staining TMEPAI in HuvEc cells by Immunofluorescence. The picture on the right is treated with the synthesized peptide.

References

This product has been referenced in:

  • de Miranda NF  et al. Transforming Growth Factor ß Signaling in Colorectal Cancer Cells With Microsatellite Instability Despite Biallelic Mutations in TGFBR2. Gastroenterology 148:1427-37.e8 (2015). Read more (PubMed: 25736321) »
See 1 Publication for this product

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