Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10671(B)] to TMF
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
Product nameAnti-TMF antibody [EPR10671(B)]
See all TMF primary antibodies
DescriptionRabbit monoclonal [EPR10671(B)] to TMF
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human TMF (C terminal).
Database link: P82094
- HepG2, HeLa, HT1376 cell lysate; MCF7 cells; HT1376 cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
Our Abpromise guarantee covers the use of ab151702 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 123 kDa.|
|ICC/IF||1/100 - 1/250.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionPotential coactivator of the androgen receptor. Mediates STAT3 degradation. May play critical roles in two RAB6-dependent retrograde transport processes: one from endosomes to the Golgi and the other from the Golgi to the ER. This protein binds the HIV-1 TATA element and inhibits transcriptional activation by the TATA-binding protein (TBP).
DomainThe Elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV].
Cellular localizationCytoplasm. Nucleus. Golgi apparatus membrane. Concentrated at the budding structures localized at the tips of cisternae.
- Information by UniProt
- Androgen receptor coactivator 160 kDa protein antibody
- Androgen receptor-associated protein of 160 kDa antibody
- ARA160 antibody
All lanes : Anti-TMF antibody [EPR10671(B)] (ab151702) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HT1376 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 123 kDa
Flow cytometric analysis of permeabilized HT1376 cells labeling TMF with ab151702 at 1/10 dilution (red). Rabbit IgG negative control (green).
Immunofluorescent analysis of MCF7 cells labeling TMF with ab151702 at 1/100 dilution.
ab151702 has not yet been referenced specifically in any publications.