Overview

  • Product name

    Anti-TMF antibody [EPR10671(B)]
    See all TMF primary antibodies
  • Description

    Rabbit monoclonal [EPR10671(B)] to TMF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human TMF (C terminal).
    Database link: P82094

  • Positive control

    • HepG2, HeLa, HT1376 cell lysate; MCF7 cells; HT1376 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab151702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 123 kDa.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Potential coactivator of the androgen receptor. Mediates STAT3 degradation. May play critical roles in two RAB6-dependent retrograde transport processes: one from endosomes to the Golgi and the other from the Golgi to the ER. This protein binds the HIV-1 TATA element and inhibits transcriptional activation by the TATA-binding protein (TBP).
    • Domain

      The Elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV].
    • Cellular localization

      Cytoplasm. Nucleus. Golgi apparatus membrane. Concentrated at the budding structures localized at the tips of cisternae.
    • Information by UniProt
    • Database links

    • Alternative names

      • Androgen receptor coactivator 160 kDa protein antibody
      • Androgen receptor-associated protein of 160 kDa antibody
      • ARA160 antibody
      • TATA element modulatory factor 1 antibody
      • TATA element modulatory factor antibody
      • TMF antibody
      • Tmf1 antibody
      • TMF1_HUMAN antibody
      see all

    Images

    • All lanes : Anti-TMF antibody [EPR10671(B)] (ab151702) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HT1376 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 123 kDa

    • Flow cytometric analysis of permeabilized HT1376 cells labeling TMF with ab151702 at 1/10 dilution (red). Rabbit IgG negative control (green).

    • Immunofluorescent analysis of MCF7 cells labeling TMF with ab151702 at 1/100 dilution.

    References

    ab151702 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (RWPE-1 - prosate epithelial cells)
    Permeabilization
    Yes - 0.2% Triton X-100
    Specification
    RWPE-1 - prosate epithelial cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde

    Dr. Armen Petrosyan

    Verified customer

    Submitted Jul 19 2019

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (22Rv1 - prostate cancer cells)
    Gel Running Conditions
    Reduced Denaturing (8% SDS-PAGE)
    Loading amount
    20 µg
    Specification
    22Rv1 - prostate cancer cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    Dr. Armen Petrosyan

    Verified customer

    Submitted Jul 18 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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