Overview

  • Product name

  • Description

    Rabbit polyclonal to TMP21
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Hamster, Cow, Orangutan
  • Immunogen

    Synthetic peptide within Human TMP21 aa 169-219. The exact sequence is proprietary.
    Database link: P49755

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 cell lysates. IP: HEK-293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab241308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 25 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Involved in vesicular protein trafficking. Mainly functions in the early secretory pathway. Thought to act as cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and to be involved in vesicle coat formation at the cytoplasmic side. In COPII vesicle-mediated anterograde transport involved in the transport of GPI-anchored proteins and proposed to act togther with TMED2 as their cargo receptor; the function specifically implies SEC24C and SEC24D of the COPII vesicle coat and lipid raft-like microdomains of the ER. Recognizes GPI anchors structural remodeled in the ER by PGAP1 and MPPE1 (By similarity). In COPI vesicle-mediated retrograde transport involved in the biogenesis of COPI vesicles and vesicle coat recruitment. On Golgi membranes, acts as primary receptor for ARF1-GDP which is involved in COPI-vesicle formation. Increases coatomer-dependent GTPase-activating activity of ARFGAP2. Involved in trafficking of G protein-coupled receptors (GPCRs). Regulates F2LR1, OPRM1 and P2RY4 exocytic trafficking from the Golgi to the plasma membrane thus contributing to receptor resensitization. Involved in trafficking of amyloid beta A4 protein and soluble APP-beta release (independent of modulation of gamma-secretase activity). As part of the presenilin-dependent gamma-secretase complex regulates gamma-cleavages of the amyloid beta A4 protein to yield amyloid-beta 40 (Abeta40). Involved in organization of the Golgi apparatus.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the EMP24/GP25L family.
    Contains 1 GOLD domain.
  • Domain

    The lumenal domain mediates localization to the plasma membrane by partially overriding the ER retention by the cytoplasmic domain.
  • Cellular localization

    Golgi apparatus > cis-Golgi network membrane. Melanosome. Endoplasmic reticulum membrane. Endoplasmic reticulum-Golgi intermediate compartment membrane. Cytoplasmic vesicle > secretory vesicle membrane. Cell membrane. Golgi apparatus > trans-Golgi network membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Cycles between compartments of the early secretatory pathway.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1110014C03Rik antibody
    • 21 kDa transmembrane trafficking protein antibody
    • 21 kDa transmembrane-trafficking protein antibody
    • MGC102351 antibody
    • p23 antibody
    • p24 family protein delta 1 antibody
    • p24 family protein delta-1 antibody
    • p24(DELTA) antibody
    • p24delta antibody
    • p24delta1 antibody
    • S31I125 antibody
    • S31III125 antibody
    • TMED 10 antibody
    • Tmed10 antibody
    • TMEDA_HUMAN antibody
    • Tmp 21 I antibody
    • Tmp 21 antibody
    • Tmp 21 I antibody
    • Tmp-21-I antibody
    • Transmembrane emp24 domain containing protein 10 antibody
    • Transmembrane emp24 domain-containing protein 10 antibody
    • Transmembrane emp24-like trafficking protein 10 (yeast) antibody
    • Transmembrane protein Tmp21 antibody
    • Transmembrane trafficking protein 21kD antibody
    see all

Images

  • All lanes : Anti-TMP21 antibody (ab241308) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 4 : TCMK-1 (mouse kidney epithelial cell line) cell lysate
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

    Lysates/proteins at 15 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 25 kDa


    Exposure time: 3 minutes


    Lysates prepared in NETN buffer.

  • TMP21 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241308 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241308 at 1 µg/ml.

    Lane 1: ab241308 IP in HEK-293T whole cell lysate.
    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with exposure time of 3 minutes.

    Lysates prepared in NETN buffer.

References

ab241308 has not yet been referenced specifically in any publications.

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