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    tmp21-antibody-ab241312.pdf

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Anti-TMP21 antibody (ab241312)

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Western blot - Anti-TMP21 antibody (ab241312)

    Key features and details

    • Rabbit polyclonal to TMP21
    • Suitable for: WB
    • Reacts with: Mouse, Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human TMP21 protein (ab167877)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-TMP21 antibody
      See all TMP21 primary antibodies
    • Description

      Rabbit polyclonal to TMP21
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse, Human
      Predicted to work with: Orangutan
    • Immunogen

      Synthetic peptide within Human TMP21 aa 50-100. The exact sequence is proprietary.
      Database link: P49755

    • Positive control

      • WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7
      Preservative: 0.09% Sodium azide
      Constituent: Tris citrate/phosphate

      pH 7-8
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      ab241312 was affinity purified using an epitope specific to TMP21 immobilized on solid support.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Alzheimer's disease
      • Other
      • Signal Transduction
      • Protein Trafficking
      • Vesicle Transport
      • Regulation

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • HeLa whole cell lysate (ab150035)
      • HeLa whole cell lysate (ab29545)
      • Jurkat whole cell lysate (ab30128)
      • NIH 3T3 whole cell lysate (ab7179)
      • Jurkat whole cell lysate (ab7899)
    • Recombinant Protein

      • Recombinant Human TMP21 protein (ab167877)
    • Related Products

      • Recombinant Human TMP21 protein (ab167877)

    Applications

    Our Abpromise guarantee covers the use of ab241312 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/2000 - 1/10000. Predicted molecular weight: 24 kDa.

     

     

    Target

    • Function

      Involved in vesicular protein trafficking. Mainly functions in the early secretory pathway. Thought to act as cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and to be involved in vesicle coat formation at the cytoplasmic side. In COPII vesicle-mediated anterograde transport involved in the transport of GPI-anchored proteins and proposed to act togther with TMED2 as their cargo receptor; the function specifically implies SEC24C and SEC24D of the COPII vesicle coat and lipid raft-like microdomains of the ER. Recognizes GPI anchors structural remodeled in the ER by PGAP1 and MPPE1 (By similarity). In COPI vesicle-mediated retrograde transport involved in the biogenesis of COPI vesicles and vesicle coat recruitment. On Golgi membranes, acts as primary receptor for ARF1-GDP which is involved in COPI-vesicle formation. Increases coatomer-dependent GTPase-activating activity of ARFGAP2. Involved in trafficking of G protein-coupled receptors (GPCRs). Regulates F2LR1, OPRM1 and P2RY4 exocytic trafficking from the Golgi to the plasma membrane thus contributing to receptor resensitization. Involved in trafficking of amyloid beta A4 protein and soluble APP-beta release (independent of modulation of gamma-secretase activity). As part of the presenilin-dependent gamma-secretase complex regulates gamma-cleavages of the amyloid beta A4 protein to yield amyloid-beta 40 (Abeta40). Involved in organization of the Golgi apparatus.
    • Tissue specificity

      Ubiquitous.
    • Sequence similarities

      Belongs to the EMP24/GP25L family.
      Contains 1 GOLD domain.
    • Domain

      The lumenal domain mediates localization to the plasma membrane by partially overriding the ER retention by the cytoplasmic domain.
    • Cellular localization

      Golgi apparatus > cis-Golgi network membrane. Melanosome. Endoplasmic reticulum membrane. Endoplasmic reticulum-Golgi intermediate compartment membrane. Cytoplasmic vesicle > secretory vesicle membrane. Cell membrane. Golgi apparatus > trans-Golgi network membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Cycles between compartments of the early secretatory pathway.
    • Target information above from: UniProt accession P49755 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 10972 Human
      • Entrez Gene: 68581 Mouse
      • Entrez Gene: 100171801 Orangutan
      • Omim: 605406 Human
      • SwissProt: P49755 Human
      • SwissProt: Q9D1D4 Mouse
      • SwissProt: Q5RE32 Orangutan
      • Unigene: 74137 Human
      • Unigene: 379159 Mouse
      • Unigene: 472063 Mouse
      see all
    • Alternative names

      • 1110014C03Rik antibody
      • 21 kDa transmembrane trafficking protein antibody
      • 21 kDa transmembrane-trafficking protein antibody
      • MGC102351 antibody
      • p23 antibody
      • p24 family protein delta 1 antibody
      • p24 family protein delta-1 antibody
      • p24(DELTA) antibody
      • p24delta antibody
      • p24delta1 antibody
      • S31I125 antibody
      • S31III125 antibody
      • TMED 10 antibody
      • Tmed10 antibody
      • TMEDA_HUMAN antibody
      • Tmp 21 I antibody
      • Tmp 21 antibody
      • Tmp 21 I antibody
      • Tmp-21-I antibody
      • Transmembrane emp24 domain containing protein 10 antibody
      • Transmembrane emp24 domain-containing protein 10 antibody
      • Transmembrane emp24-like trafficking protein 10 (yeast) antibody
      • Transmembrane protein Tmp21 antibody
      • Transmembrane trafficking protein 21kD antibody
      see all

    Images

    • Western blot - Anti-TMP21 antibody (ab241312)
      Western blot - Anti-TMP21 antibody (ab241312)
      All lanes : Anti-TMP21 antibody (ab241312) at 0.1 µg/ml

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
      Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
      Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate

      Lysates/proteins at 15 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 24 kDa


      Exposure time: 30 seconds


      Lysates prepared using NETN lysis buffer.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab241312? Please let us know so that we can cite the reference in this datasheet.

    ab241312 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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