Overview

  • Product name

    Anti-TMP21 antibody - C-terminal
    See all TMP21 primary antibodies
  • Description

    Rabbit polyclonal to TMP21 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human TMP21 (C terminal). NCBI Accession No. NP_006818.3. The synthetic peptide corresponds to 19 amino acids.
    Database link: P49755

  • Positive control

    • Human breast and prostate tissues; Raji cell lysate; Human brain cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab189088 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 25 kDa.
IHC-P Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Involved in vesicular protein trafficking. Mainly functions in the early secretory pathway. Thought to act as cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and to be involved in vesicle coat formation at the cytoplasmic side. In COPII vesicle-mediated anterograde transport involved in the transport of GPI-anchored proteins and proposed to act togther with TMED2 as their cargo receptor; the function specifically implies SEC24C and SEC24D of the COPII vesicle coat and lipid raft-like microdomains of the ER. Recognizes GPI anchors structural remodeled in the ER by PGAP1 and MPPE1 (By similarity). In COPI vesicle-mediated retrograde transport involved in the biogenesis of COPI vesicles and vesicle coat recruitment. On Golgi membranes, acts as primary receptor for ARF1-GDP which is involved in COPI-vesicle formation. Increases coatomer-dependent GTPase-activating activity of ARFGAP2. Involved in trafficking of G protein-coupled receptors (GPCRs). Regulates F2LR1, OPRM1 and P2RY4 exocytic trafficking from the Golgi to the plasma membrane thus contributing to receptor resensitization. Involved in trafficking of amyloid beta A4 protein and soluble APP-beta release (independent of modulation of gamma-secretase activity). As part of the presenilin-dependent gamma-secretase complex regulates gamma-cleavages of the amyloid beta A4 protein to yield amyloid-beta 40 (Abeta40). Involved in organization of the Golgi apparatus.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the EMP24/GP25L family.
    Contains 1 GOLD domain.
  • Domain

    The lumenal domain mediates localization to the plasma membrane by partially overriding the ER retention by the cytoplasmic domain.
  • Cellular localization

    Golgi apparatus > cis-Golgi network membrane. Melanosome. Endoplasmic reticulum membrane. Endoplasmic reticulum-Golgi intermediate compartment membrane. Cytoplasmic vesicle > secretory vesicle membrane. Cell membrane. Golgi apparatus > trans-Golgi network membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Cycles between compartments of the early secretatory pathway.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1110014C03Rik antibody
    • 21 kDa transmembrane trafficking protein antibody
    • 21 kDa transmembrane-trafficking protein antibody
    • MGC102351 antibody
    • p23 antibody
    • p24 family protein delta 1 antibody
    • p24 family protein delta-1 antibody
    • p24(DELTA) antibody
    • p24delta antibody
    • p24delta1 antibody
    • S31I125 antibody
    • S31III125 antibody
    • TMED 10 antibody
    • Tmed10 antibody
    • TMEDA_HUMAN antibody
    • Tmp 21 I antibody
    • Tmp 21 antibody
    • Tmp 21 I antibody
    • Tmp-21-I antibody
    • Transmembrane emp24 domain containing protein 10 antibody
    • Transmembrane emp24 domain-containing protein 10 antibody
    • Transmembrane emp24-like trafficking protein 10 (yeast) antibody
    • Transmembrane protein Tmp21 antibody
    • Transmembrane trafficking protein 21kD antibody
    see all

Images

  • Lane 1 : Anti-TMP21 antibody - C-terminal (ab189088) at 0.5 µg/ml
    Lane 2 : Anti-TMP21 antibody - C-terminal (ab189088) at 1 µg/ml
    Lane 3 : Anti-TMP21 antibody - C-terminal (ab189088) at 2 µg/ml

    All lanes : Raji cell lysate

    Predicted band size: 25 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast tissue, labeling TMP21 using ab189088 at 3 µg/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate tissue, labeling TMP21 using ab189088 at 3 µg/ml.

  • Immunofluorescence analysis of human brain cells labeling TMP21 using ab189088 at 20 µg/ml.

References

ab189088 has not yet been referenced specifically in any publications.

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