Key features and details
- Rabbit polyclonal to TMP21 - C-terminal
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-TMP21 antibody - C-terminal
See all TMP21 primary antibodies
DescriptionRabbit polyclonal to TMP21 - C-terminal
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human TMP21 (C terminal). NCBI Accession No. NP_006818.3. The synthetic peptide corresponds to 19 amino acids.
Database link: P49755
- Human breast and prostate tissues; Raji cell lysate; Human brain cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab189088 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 25 kDa.|
|IHC-P||Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionInvolved in vesicular protein trafficking. Mainly functions in the early secretory pathway. Thought to act as cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and to be involved in vesicle coat formation at the cytoplasmic side. In COPII vesicle-mediated anterograde transport involved in the transport of GPI-anchored proteins and proposed to act togther with TMED2 as their cargo receptor; the function specifically implies SEC24C and SEC24D of the COPII vesicle coat and lipid raft-like microdomains of the ER. Recognizes GPI anchors structural remodeled in the ER by PGAP1 and MPPE1 (By similarity). In COPI vesicle-mediated retrograde transport involved in the biogenesis of COPI vesicles and vesicle coat recruitment. On Golgi membranes, acts as primary receptor for ARF1-GDP which is involved in COPI-vesicle formation. Increases coatomer-dependent GTPase-activating activity of ARFGAP2. Involved in trafficking of G protein-coupled receptors (GPCRs). Regulates F2LR1, OPRM1 and P2RY4 exocytic trafficking from the Golgi to the plasma membrane thus contributing to receptor resensitization. Involved in trafficking of amyloid beta A4 protein and soluble APP-beta release (independent of modulation of gamma-secretase activity). As part of the presenilin-dependent gamma-secretase complex regulates gamma-cleavages of the amyloid beta A4 protein to yield amyloid-beta 40 (Abeta40). Involved in organization of the Golgi apparatus.
Sequence similaritiesBelongs to the EMP24/GP25L family.
Contains 1 GOLD domain.
DomainThe lumenal domain mediates localization to the plasma membrane by partially overriding the ER retention by the cytoplasmic domain.
Cellular localizationGolgi apparatus > cis-Golgi network membrane. Melanosome. Endoplasmic reticulum membrane. Endoplasmic reticulum-Golgi intermediate compartment membrane. Cytoplasmic vesicle > secretory vesicle membrane. Cell membrane. Golgi apparatus > trans-Golgi network membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Cycles between compartments of the early secretatory pathway.
- Information by UniProt
- 1110014C03Rik antibody
- 21 kDa transmembrane trafficking protein antibody
- 21 kDa transmembrane-trafficking protein antibody
Lane 1 : Anti-TMP21 antibody - C-terminal (ab189088) at 0.5 µg/ml
Lane 2 : Anti-TMP21 antibody - C-terminal (ab189088) at 1 µg/ml
Lane 3 : Anti-TMP21 antibody - C-terminal (ab189088) at 2 µg/ml
All lanes : Raji cell lysate
Predicted band size: 25 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast tissue, labeling TMP21 using ab189088 at 3 µg/ml.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate tissue, labeling TMP21 using ab189088 at 3 µg/ml.
Immunofluorescence analysis of human brain cells labeling TMP21 using ab189088 at 20 µg/ml.
ab189088 has not yet been referenced specifically in any publications.