Overview

  • Product name

    Anti-TMP21 antibody [EPR9037(B)]
    See all TMP21 primary antibodies
  • Description

    Rabbit monoclonal [EPR9037(B)] to TMP21
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human TMP21 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • U-87 MG, Raji, HeLa, 293T, C6, RAW 264.7, PC12 and NIH 3T3 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 19 kDa (predicted molecular weight: 25 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Involved in vesicular protein trafficking. Mainly functions in the early secretory pathway. Thought to act as cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and to be involved in vesicle coat formation at the cytoplasmic side. In COPII vesicle-mediated anterograde transport involved in the transport of GPI-anchored proteins and proposed to act togther with TMED2 as their cargo receptor; the function specifically implies SEC24C and SEC24D of the COPII vesicle coat and lipid raft-like microdomains of the ER. Recognizes GPI anchors structural remodeled in the ER by PGAP1 and MPPE1 (By similarity). In COPI vesicle-mediated retrograde transport involved in the biogenesis of COPI vesicles and vesicle coat recruitment. On Golgi membranes, acts as primary receptor for ARF1-GDP which is involved in COPI-vesicle formation. Increases coatomer-dependent GTPase-activating activity of ARFGAP2. Involved in trafficking of G protein-coupled receptors (GPCRs). Regulates F2LR1, OPRM1 and P2RY4 exocytic trafficking from the Golgi to the plasma membrane thus contributing to receptor resensitization. Involved in trafficking of amyloid beta A4 protein and soluble APP-beta release (independent of modulation of gamma-secretase activity). As part of the presenilin-dependent gamma-secretase complex regulates gamma-cleavages of the amyloid beta A4 protein to yield amyloid-beta 40 (Abeta40). Involved in organization of the Golgi apparatus.
    • Tissue specificity

      Ubiquitous.
    • Sequence similarities

      Belongs to the EMP24/GP25L family.
      Contains 1 GOLD domain.
    • Domain

      The lumenal domain mediates localization to the plasma membrane by partially overriding the ER retention by the cytoplasmic domain.
    • Cellular localization

      Golgi apparatus > cis-Golgi network membrane. Melanosome. Endoplasmic reticulum membrane. Endoplasmic reticulum-Golgi intermediate compartment membrane. Cytoplasmic vesicle > secretory vesicle membrane. Cell membrane. Golgi apparatus > trans-Golgi network membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Cycles between compartments of the early secretatory pathway.
    • Information by UniProt
    • Database links

    • Alternative names

      • 1110014C03Rik antibody
      • 21 kDa transmembrane trafficking protein antibody
      • 21 kDa transmembrane-trafficking protein antibody
      • MGC102351 antibody
      • p23 antibody
      • p24 family protein delta 1 antibody
      • p24 family protein delta-1 antibody
      • p24(DELTA) antibody
      • p24delta antibody
      • p24delta1 antibody
      • S31I125 antibody
      • S31III125 antibody
      • TMED 10 antibody
      • Tmed10 antibody
      • TMEDA_HUMAN antibody
      • Tmp 21 I antibody
      • Tmp 21 antibody
      • Tmp 21 I antibody
      • Tmp-21-I antibody
      • Transmembrane emp24 domain containing protein 10 antibody
      • Transmembrane emp24 domain-containing protein 10 antibody
      • Transmembrane emp24-like trafficking protein 10 (yeast) antibody
      • Transmembrane protein Tmp21 antibody
      • Transmembrane trafficking protein 21kD antibody
      see all

    Images

    • All lanes : Anti-TMP21 antibody [EPR9037(B)] (ab133771) at 1/1000 dilution

      Lane 1 : U-87 MG cell lysate
      Lane 2 : Raji cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : 293T cell lysate
      Lane 5 : C6 cell lysate
      Lane 6 : RAW 264.7 cell lysate
      Lane 7 : PC12 cell lysate
      Lane 8 : NIH 3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

      Predicted band size: 25 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    See 1 Publication for this product

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