Overview

  • Product name

  • Description

    Rabbit polyclonal to TMPRSS11D
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 152-348 of Human TMPRSS11D (UniProt O60235).

  • Positive control

    • HepG2 whole cell lysate (ab7900); Human breast carcinoma tissue
  • General notes

    Store undiluted.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab127031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 46 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    May play some biological role in the host defense system on the mucous membrane independently of or in cooperation with other substances in airway mucous or bronchial secretions.
  • Tissue specificity

    Located in the cells of the submucosal serous glands of the bronchi and trachea.
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
    Contains 1 SEA domain.
  • Cellular localization

    Secreted. Activated by cleavage and secreted and Cell membrane. Activated by cleavage and secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Airway trypsin like protease antibody
    • Airway trypsin-like protease antibody
    • HAT antibody
    • MGC150587 antibody
    • MGC150588 antibody
    • TM11D_HUMAN antibody
    • TMPRSS11D antibody
    • Transmembrane protease serine 11D antibody
    • Transmembrane protease serine 11D catalytic chain antibody
    • Transmembrane protease serine 11D precursor antibody
    see all

Images

  • Anti-TMPRSS11D antibody (ab127031) at 1/500 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 46 kDa



    10% SDS-PAGE
  • ab127031, at 1/250 dilution, staining TMPRSS11D in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.

References

This product has been referenced in:

  • Muytjens CMJ  et al. Functional proteomic profiling reveals KLK13 and TMPRSS11D as active proteases in the lower female reproductive tract. F1000Res 7:1666 (2018). Read more (PubMed: 30647911) »
  • Cao X  et al. High TMPRSS11D protein expression predicts poor overall survival in non-small cell lung cancer. Oncotarget 8:12812-12819 (2017). Read more (PubMed: 28086212) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12,5%)
Sample
Monkey Cell lysate - whole cell (293T HEK transfected with macaque TMPRSS11D)
Specification
293T HEK transfected with macaque TMPRSS11D
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

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Verified customer

Submitted Jan 31 2014

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