Key features and details
- Rabbit polyclonal to TMPRSS2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-TMPRSS2 antibody
See all TMPRSS2 primary antibodies
DescriptionRabbit polyclonal to TMPRSS2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Synthetic peptide corresponding to Human TMPRSS2 aa 320-370 conjugated to keyhole limpet haemocyanin.
Database link: O15393
- WB: LnCaP, Caco 2 and Du145 cell lysates; Human prostate, lung, heart and brain tissues. IHC: Mouse lung tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab214462 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/1000. Predicted molecular weight: 54 kDa.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Tissue specificityExpressed strongly in small intestine. Also expressed in prostate, colon, stomach and salivary gland.
Sequence similaritiesBelongs to the peptidase S1 family.
Contains 1 LDL-receptor class A domain.
Contains 1 peptidase S1 domain.
Contains 1 SRCR domain.
Cellular localizationCell membrane and Secreted. Activated by cleavage and secreted.
- Information by UniProt
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All lanes : Anti-TMPRSS2 antibody (ab214462) at 1/500 dilution
Lane 1 : LnCaP cell lysate
Lane 2 : Human prostate tissue lysate
Lane 3 : Caco 2 cell lysate
Lane 4 : Human lung tissue lysate
Lane 5 : Human heart tissue lysate
Lane 6 : Human brain tissue lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Lanes 1 - 6: Merged signal (red and green). Green - ab214462 observed at 52 kDa. Red - loading control, mouse anti GAPDH observed at 37kDa.
ab214462 was shown to react with TMPRSS2 in western blot. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab214462 and mouse anti GAPDH overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Anti-TMPRSS2 antibody (ab214462) at 1/300 dilution + Du145 cell lysate
secondary antibody HRP-conjugated at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 54 kDa
Immunohistochemical analysis of formalin-fixed and paraffin-embedded mouse lung tissue labeling TMPRSS2 with ab214462 at 1/100 dilution, followed by conjugation to the secondary antibody and DAB staining.
ab214462 has not yet been referenced specifically in any publications.