Recombinant
RabMAb

Recombinant Anti-TMPRSS2 antibody [EPR3861] - BSA and Azide free (ab239905)

Overview

  • Product name

    Anti-TMPRSS2 antibody [EPR3861] - BSA and Azide free
    See all TMPRSS2 primary antibodies
  • Description

    Rabbit monoclonal [EPR3861] to TMPRSS2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Flow Cyt, ICC, WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Cynomolgus monkey, Rhesus monkey, Common marmoset
  • Immunogen

    Synthetic peptide within Human TMPRSS2 aa 1-100. The exact sequence is proprietary.

  • General notes

    Ab239905 is the carrier-free version of ab92323. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab239905 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab239905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

The use of an HRP/AP polymerized secondary antibody is recommended as stronger signals have been found using these.

 

Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 54 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Tissue specificity

      Expressed strongly in small intestine. Also expressed in prostate, colon, stomach and salivary gland.
    • Sequence similarities

      Belongs to the peptidase S1 family.
      Contains 1 LDL-receptor class A domain.
      Contains 1 peptidase S1 domain.
      Contains 1 SRCR domain.
    • Cellular localization

      Cell membrane and Secreted. Activated by cleavage and secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • D16Ertd61e antibody
      • Epitheliasin antibody
      • FLJ41954 antibody
      • MGC6821 antibody
      • PP9284 antibody
      • PRSS10 antibody
      • Serine protease 10 antibody
      • TMPRSS2 antibody
      • TMPRSS2 ERG FUSION GENE, INCLUDED antibody
      • TMPRSS2 ETV1 FUSION GENE, INCLUDED antibody
      • TMPS2_HUMAN antibody
      • Transmembrane protease serine 2 catalytic chain antibody
      • Transmembrane protease, serine 2 antibody
      • Transmembrane protease, serine 2, EC 3.4.219 antibody
      see all

    Images

    • ab92323 at 1/1000 dilution, staining TMPRSS2 in paraffin embedded Human prostatic adenocarcinoma

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92323).

    • Overlay histogram showing LoVo cells stained with ab92323 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92323, 1µg/1x106 cells) for 30 min at 22�C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22�C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

      Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92323).

    References

    ab239905 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab239905.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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