Product nameAnti-TMPRSS2 antibody [EPR3862]
See all TMPRSS2 primary antibodies
DescriptionRabbit monoclonal [EPR3862] to TMPRSS2
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human TMPRSS2 aa 450-550 (C terminal). The exact sequence is proprietary.
- Human prostate tissue lysate, Human prostatic adenocarcinoma tissue
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab109131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 54,28 kDa (predicted molecular weight: 54 kDa).|
|IHC-P||1/1000 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For unpurified use at 1/500 - 1/1000.
Tissue specificityExpressed strongly in small intestine. Also expressed in prostate, colon, stomach and salivary gland.
Sequence similaritiesBelongs to the peptidase S1 family.
Contains 1 LDL-receptor class A domain.
Contains 1 peptidase S1 domain.
Contains 1 SRCR domain.
Cellular localizationCell membrane and Secreted. Activated by cleavage and secreted.
- Information by UniProt
- D16Ertd61e antibody
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ab109131 staining TMPRSS2 in Human prostate adenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/1000). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.
ab109131 staining TMPRSS2 in the HT-29 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/150). ab150077(1/500) an Alexa Fluor®488-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI.
All lanes : Anti-TMPRSS2 antibody [EPR3862] (ab109131) at 1/5000 dilution
Lane 1 : Human prostrate Lysate
Lane 2 : LnCaP Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 54 kDa
All lanes : Anti-TMPRSS2 antibody [EPR3862] (ab109131) at 1/1000 dilution (unpurified)
All lanes : Human prostate tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 54 kDa
Immunohistochemical analysis of paraffin-embedded Human prostatic adenocarcinoma using ab109131, unpurified, at a dilution of 1/500.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ab109131 has not yet been referenced specifically in any publications.