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    tmprss2-antibody-epr3862-bsa-and-azide-free-ab242384.pdf

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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-TMPRSS2 antibody [EPR3862] - BSA and Azide free (ab242384)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-TMPRSS2 antibody [EPR3862] - BSA and Azide free (ab242384)
  • Western blot - Anti-TMPRSS2 antibody [EPR3862] - BSA and Azide free (ab242384)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS2 antibody [EPR3862] - BSA and Azide free (ab242384)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS2 antibody [EPR3862] - BSA and Azide free (ab242384)
  • Anti-TMPRSS2 antibody [EPR3862] - BSA and Azide free (ab242384)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3862] to TMPRSS2 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

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Overview

  • Product name

    Anti-TMPRSS2 antibody [EPR3862] - BSA and Azide free
    See all TMPRSS2 primary antibodies
  • Description

    Rabbit monoclonal [EPR3862] to TMPRSS2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human prostatic adenocarcinoma tissue; WB: Human prostate lysate.
  • General notes

    ab242384 is the carrier-free version of ab109131.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3862
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Serine protease
    • TMPRSS
    • Cancer
    • Tumor biomarkers
    • Enzymes
    • Other

Associated products

  • Alternative Versions

    • Anti-TMPRSS2 antibody [EPR3862] (ab109131)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Conjugation kits

    • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
  • Recombinant Protein

    • Recombinant Human TMPRSS2 protein (ab112364)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab242384 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 54,28 kDa (predicted molecular weight: 54 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 54,28 kDa (predicted molecular weight: 54 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Application notes
Is unsuitable for ICC.

Target

  • Tissue specificity

    Expressed strongly in small intestine. Also expressed in prostate, colon, stomach and salivary gland.
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 1 LDL-receptor class A domain.
    Contains 1 peptidase S1 domain.
    Contains 1 SRCR domain.
  • Cellular localization

    Cell membrane and Secreted. Activated by cleavage and secreted.
  • Target information above from: UniProt accession O15393 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7113 Human
    • Omim: 602060 Human
    • SwissProt: O15393 Human
    • Unigene: 439309 Human
    • Alternative names

      • D16Ertd61e antibody
      • Epitheliasin antibody
      • FLJ41954 antibody
      • MGC6821 antibody
      • PP9284 antibody
      • PRSS10 antibody
      • Serine protease 10 antibody
      • TMPRSS2 antibody
      • TMPRSS2 ERG FUSION GENE, INCLUDED antibody
      • TMPRSS2 ETV1 FUSION GENE, INCLUDED antibody
      • TMPS2_HUMAN antibody
      • Transmembrane protease serine 2 catalytic chain antibody
      • Transmembrane protease, serine 2 antibody
      • Transmembrane protease, serine 2, EC 3.4.219 antibody
      see all

    Images

    • Western blot - Anti-TMPRSS2 antibody [EPR3862] - BSA and Azide free (ab242384)
      Western blot - Anti-TMPRSS2 antibody [EPR3862] - BSA and Azide free (ab242384)
      All lanes : Anti-TMPRSS2 antibody [EPR3862] (ab109131) at 1/2000 dilution

      Lane 1 : Wild-type LNCaP cell lysate
      Lane 2 : TMPRSS2 knockout LNCaP cell lysate
      Lane 3 : Wild-type Caco-2 cell lysate
      Lane 4 : TMPRSS2 knockout Caco-2 cell lysate
      Lane 5 : PC-3 cell lysate
      Lane 6 : DU 145 cell lysate
      Lane 7 : Human Prostate cell lysate
      Lane 8 : Human Colon cell lysate
      Lane 9 : Human Lung cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 54 kDa
      Observed band size: 25, 55 kDa why is the actual band size different from the predicted?



      False colour image of Western blot: Anti-TMPRSS2 antibody [EPR3862] staining at 1/2000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab109131 was shown to bind specifically to TMPRSS2. A band was observed at 55, 25 kDa in wild-type LNCaP and at 25 kDa in Caco-2 cell lysates with no signal observed at this size in TMPRSS2 knockout LNCaP cell line ab273745 (knockout LNCaP cell lysate ab275499) and TMPRSS2 knockout Caco-2 cell line ab273737 (knockout Caco-2 cell lysate ab277340). To generate this image, wild-type and TMPRSS2 knockout cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye$®$ 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

    • Western blot - Anti-TMPRSS2 antibody [EPR3862] - BSA and Azide free (ab242384)
      Western blot - Anti-TMPRSS2 antibody [EPR3862] - BSA and Azide free (ab242384)
      Anti-TMPRSS2 antibody [EPR3862] (ab109131) at 1/5000 dilution (Purified) + Human prostate lysate

      Secondary
      Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 54 kDa



      54kDa represents full-length TMPRSS2 and 25kDa is the cleaved form. The molecular weights observed are consistent with what have been described in the literature (PMID: 11245484).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS2 antibody [EPR3862] - BSA and Azide free (ab242384)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS2 antibody [EPR3862] - BSA and Azide free (ab242384)

      This data was developed using ab109131, the same antibody clone in a different buffer formulation.

      ab109131 staining TMPRSS2 in Human prostate adenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/1000). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS2 antibody [EPR3862] - BSA and Azide free (ab242384)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS2 antibody [EPR3862] - BSA and Azide free (ab242384)

      This data was developed using ab109131, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human prostatic adenocarcinoma using ab109131, unpurified, at a dilution of 1/500.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-TMPRSS2 antibody [EPR3862] - BSA and Azide free (ab242384)
      Anti-TMPRSS2 antibody [EPR3862] - BSA and Azide free (ab242384)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (2)

    Publishing research using ab242384? Please let us know so that we can cite the reference in this datasheet.

    ab242384 has been referenced in 2 publications.

    • Zhao Y  et al. Type 2 diabetes mellitus impaired nasal immunity and increased the risk of hyposmia in COVID-19 mild pneumonia patients. Int Immunopharmacol 93:107406 (2021). PubMed: 33601246
    • Eriksen AZ  et al. SARS-CoV-2 infects human adult donor eyes and hESC-derived ocular epithelium. Cell Stem Cell 28:1205-1220.e7 (2021). PubMed: 34022129

    Customer reviews and Q&As

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