Recombinant Anti-TMPRSS2 antibody [EPR3862] - BSA and Azide free (ab242384)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3862] to TMPRSS2 - BSA and Azide free
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TMPRSS2 antibody [EPR3862] - BSA and Azide free
See all TMPRSS2 primary antibodies -
Description
Rabbit monoclonal [EPR3862] to TMPRSS2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human prostatic adenocarcinoma tissue; WB: Human prostate lysate.
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General notes
ab242384 is the carrier-free version of ab109131.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3862 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-TMPRSS2 antibody [EPR3862] (ab109131)
- Alexa Fluor® 488 Anti-TMPRSS2 antibody [EPR3862] (ab311034)
- Alexa Fluor® 647 Anti-TMPRSS2 antibody [EPR3862] (ab311160)
- Alexa Fluor® 594 Anti-TMPRSS2 antibody [EPR3862] (ab311809)
- Alexa Fluor® 568 Anti-TMPRSS2 antibody [EPR3862] (ab313092)
- Alexa Fluor® 555 Anti-TMPRSS2 antibody [EPR3862] (ab313286)
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Compatible Secondaries
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Conjugation kits
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab242384 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 54,28 kDa (predicted molecular weight: 54 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 54,28 kDa (predicted molecular weight: 54 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Tissue specificity
Expressed strongly in small intestine. Also expressed in prostate, colon, stomach and salivary gland. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 LDL-receptor class A domain.
Contains 1 peptidase S1 domain.
Contains 1 SRCR domain. -
Cellular localization
Cell membrane and Secreted. Activated by cleavage and secreted. - Information by UniProt
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Database links
- Entrez Gene: 7113 Human
- Omim: 602060 Human
- SwissProt: O15393 Human
- Unigene: 439309 Human
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Alternative names
- D16Ertd61e antibody
- Epitheliasin antibody
- FLJ41954 antibody
see all
Images
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All lanes : Anti-TMPRSS2 antibody [EPR3862] (ab109131) at 1/2000 dilution
Lane 1 : Wild-type LNCaP cell lysate
Lane 2 : TMPRSS2 knockout LNCaP cell lysate
Lane 3 : Wild-type Caco-2 cell lysate
Lane 4 : TMPRSS2 knockout Caco-2 cell lysate
Lane 5 : PC-3 cell lysate
Lane 6 : DU 145 cell lysate
Lane 7 : Human Prostate cell lysate
Lane 8 : Human Colon cell lysate
Lane 9 : Human Lung cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 25, 55 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-TMPRSS2 antibody [EPR3862] staining at 1/2000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab109131 was shown to bind specifically to TMPRSS2. A band was observed at 55, 25 kDa in wild-type LNCaP and at 25 kDa in Caco-2 cell lysates with no signal observed at this size in TMPRSS2 knockout LNCaP cell line ab273745 (knockout LNCaP cell lysate ab275499) and TMPRSS2 knockout Caco-2 cell line ab273737 (knockout Caco-2 cell lysate ab277340). To generate this image, wild-type and TMPRSS2 knockout cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye$®$ 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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Anti-TMPRSS2 antibody [EPR3862] (ab109131) at 1/5000 dilution (Purified) + Human prostate lysate
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 54 kDa54kDa represents full-length TMPRSS2 and 25kDa is the cleaved form. The molecular weights observed are consistent with what have been described in the literature (PMID: 11245484).
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This data was developed using ab109131, the same antibody clone in a different buffer formulation.
ab109131 staining TMPRSS2 in Human prostate adenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/1000). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.
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This data was developed using ab109131, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human prostatic adenocarcinoma using ab109131, unpurified, at a dilution of 1/500.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (3)
ab242384 has been referenced in 3 publications.
- Eriksen AZ et al. Protocols for SARS-CoV-2 infection in primary ocular cells and eye organoids. STAR Protoc 3:101383 (2022). PubMed: 35664254
- Zhao Y et al. Type 2 diabetes mellitus impaired nasal immunity and increased the risk of hyposmia in COVID-19 mild pneumonia patients. Int Immunopharmacol 93:107406 (2021). PubMed: 33601246
- Eriksen AZ et al. SARS-CoV-2 infects human adult donor eyes and hESC-derived ocular epithelium. Cell Stem Cell 28:1205-1220.e7 (2021). PubMed: 34022129