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RecombinantRabMAb

Recombinant Anti-TMPRSS3 antibody [EPR7048] (ab167160)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
  • Western blot - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
  • Immunocytochemistry/ Immunofluorescence - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
  • Anti-TMPRSS3 antibody [EPR7048] (ab167160)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7048] to TMPRSS3
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Rat, Human

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Overview

  • Product name

    Anti-TMPRSS3 antibody [EPR7048]
    See all TMPRSS3 primary antibodies
  • Description

    Rabbit monoclonal [EPR7048] to TMPRSS3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human TMPRSS3 (extracellular). The exact sequence is proprietary.

  • Positive control

    • HeLa, PC-12 and A431 cell lysates. Human thyroid gland papillary carcinoma tissue. A431 cells.
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7048
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Serine protease
    • TMPRSS

Associated products

  • Alternative Versions

    • Anti-TMPRSS3 antibody [EPR7048] - BSA and Azide free (ab249403)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • A431 whole cell lysate (ab30132)
    • A431 whole cell lysate (ab7909)

Applications

Our Abpromise guarantee covers the use of ab167160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 49 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Probable serine protease that play a role in hearing. Acts as a permissive factor for cochlear hair cells survival and activation at the onset of hearing and is required for saccular hair cell survival (By similarity). Activates ENaC (in vitro).
    • Tissue specificity

      Expressed in many tissues including fetal cochlea. Isoform T is found at increased levels in some carcinomas.
    • Involvement in disease

      Defects in TMPRSS3 are the cause of deafness autosomal recessive type 8 (DFNB8) [MIM:601072]. DFNA8 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
      Defects in TMPRSS3 are the cause of deafness autosomal recessive type 10 (DFNB10) [MIM:605316].
    • Sequence similarities

      Belongs to the peptidase S1 family.
      Contains 1 LDL-receptor class A domain.
      Contains 1 peptidase S1 domain.
      Contains 1 SRCR domain.
    • Post-translational
      modifications

      Undergoes autoproteolytic activation.
    • Cellular localization

      Endoplasmic reticulum membrane.
    • Target information above from: UniProt accession P57727 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 64699 Human
      • Entrez Gene: 309665 Rat
      • Omim: 605511 Human
      • SwissProt: P57727 Human
      • Unigene: 208600 Human
      • Unigene: 78889 Rat
      • Alternative names

        • Deafness autosomal recessive 10 antibody
        • DFNB10 antibody
        • DFNB8 antibody
        • ECHOS1 antibody
        • Gene similar to transmembrane serine protease antibody
        • MGC130589 antibody
        • Serine protease TADG-12 antibody
        • si:dz69g10.3 antibody
        • TADG12 antibody
        • TMPRSS 3 antibody
        • TMPRSS3 antibody
        • TMPS3_HUMAN antibody
        • Transmembrane protease serine 3 antibody
        • Tumor associated differentially expressed gene 12 protein antibody
        • Tumor-associated differentially-expressed gene 12 protein antibody
        • UNQ323/PRO382 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)

        ab167160 showing +ve staining in Human cervical carcinoma.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)

        ab167160 showing +ve staining in Human normal tonsil.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)

        ab167160 showing +ve staining in Human lung adenocarcinoma.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)

        ab167160 showing +ve staining in Human normal kidney.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)

        ab167160 showing +ve staining in Human normal colon.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)

        ab167160 showing +ve staining in Human breast carcinoma.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Western blot - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
        Western blot - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
        All lanes : Anti-TMPRSS3 antibody [EPR7048] (ab167160) at 1/1000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : PC-12 cell lysate
        Lane 3 : A431 cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 49 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)

        Immunohistochemical analysis of paraffin embedded Human thyroid gland papillary carcinoma tissue labeling TMPRSS3 with ab167160 at a 1/100 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunocytochemistry/ Immunofluorescence - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
        Immunocytochemistry/ Immunofluorescence - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
        Immunocytochemical analysis of A431 cells labeling TMPRSS3 with ab167160 at a 1/250 dilution.
      • Anti-TMPRSS3 antibody [EPR7048] (ab167160)
        Anti-TMPRSS3 antibody [EPR7048] (ab167160)

      Protocols

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab167160? Please let us know so that we can cite the reference in this datasheet.

      ab167160 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      There are currently no Customer reviews or Questions for ab167160.
      Please use the links above to contact us or submit feedback about this product.

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