Recombinant Anti-TMPRSS3 antibody [EPR7048] (ab167160)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7048] to TMPRSS3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
Overview
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Product name
Anti-TMPRSS3 antibody [EPR7048]
See all TMPRSS3 primary antibodies -
Description
Rabbit monoclonal [EPR7048] to TMPRSS3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide within Human TMPRSS3 (extracellular). The exact sequence is proprietary.
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Positive control
- HeLa, PC-12 and A431 cell lysates. Human thyroid gland papillary carcinoma tissue. A431 cells.
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General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7048 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab167160 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/1000 - 1/10000. Predicted molecular weight: 49 kDa. | |
IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
ICC/IF | 1/250 - 1/500. |
Target
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Function
Probable serine protease that play a role in hearing. Acts as a permissive factor for cochlear hair cells survival and activation at the onset of hearing and is required for saccular hair cell survival (By similarity). Activates ENaC (in vitro). -
Tissue specificity
Expressed in many tissues including fetal cochlea. Isoform T is found at increased levels in some carcinomas. -
Involvement in disease
Defects in TMPRSS3 are the cause of deafness autosomal recessive type 8 (DFNB8) [MIM:601072]. DFNA8 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
Defects in TMPRSS3 are the cause of deafness autosomal recessive type 10 (DFNB10) [MIM:605316]. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 LDL-receptor class A domain.
Contains 1 peptidase S1 domain.
Contains 1 SRCR domain. -
Post-translational
modificationsUndergoes autoproteolytic activation. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 64699 Human
- Entrez Gene: 309665 Rat
- Omim: 605511 Human
- SwissProt: P57727 Human
- Unigene: 208600 Human
- Unigene: 78889 Rat
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Alternative names
- Deafness autosomal recessive 10 antibody
- DFNB10 antibody
- DFNB8 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
ab167160 showing +ve staining in Human cervical carcinoma.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
ab167160 showing +ve staining in Human normal tonsil.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
ab167160 showing +ve staining in Human lung adenocarcinoma.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
ab167160 showing +ve staining in Human normal kidney.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
ab167160 showing +ve staining in Human normal colon.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
ab167160 showing +ve staining in Human breast carcinoma.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-TMPRSS3 antibody [EPR7048] (ab167160) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : PC-12 cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 49 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
Immunohistochemical analysis of paraffin embedded Human thyroid gland papillary carcinoma tissue labeling TMPRSS3 with ab167160 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemical analysis of A431 cells labeling TMPRSS3 with ab167160 at a 1/250 dilution.
Protocols
Datasheets and documents
References (0)
ab167160 has not yet been referenced specifically in any publications.