Recombinant
RabMAb

Recombinant Anti-TMPRSS3 antibody [EPR7048] (ab167160)

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Overview

  • Product name

    Anti-TMPRSS3 antibody [EPR7048]
    See all TMPRSS3 primary antibodies

  • Description

    Rabbit monoclonal [EPR7048] to TMPRSS3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide within Human TMPRSS3 (extracellular). The exact sequence is proprietary.

  • Positive control

    • HeLa, PC-12 and A431 cell lysates. Human thyroid gland papillary carcinoma tissue. A431 cells.

  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab167160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 49 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Probable serine protease that play a role in hearing. Acts as a permissive factor for cochlear hair cells survival and activation at the onset of hearing and is required for saccular hair cell survival (By similarity). Activates ENaC (in vitro).

    • Tissue specificity

      Expressed in many tissues including fetal cochlea. Isoform T is found at increased levels in some carcinomas.

    • Involvement in disease

      Defects in TMPRSS3 are the cause of deafness autosomal recessive type 8 (DFNB8) [MIM:601072]. DFNA8 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
      Defects in TMPRSS3 are the cause of deafness autosomal recessive type 10 (DFNB10) [MIM:605316].

    • Sequence similarities

      Belongs to the peptidase S1 family.
      Contains 1 LDL-receptor class A domain.
      Contains 1 peptidase S1 domain.
      Contains 1 SRCR domain.

    • Post-translational
      modifications

      Undergoes autoproteolytic activation.

    • Cellular localization

      Endoplasmic reticulum membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • Deafness autosomal recessive 10 antibody
        • DFNB10 antibody
        • DFNB8 antibody
        • ECHOS1 antibody
        • Gene similar to transmembrane serine protease antibody
        • MGC130589 antibody
        • Serine protease TADG-12 antibody
        • si:dz69g10.3 antibody
        • TADG12 antibody
        • TMPRSS 3 antibody
        • TMPRSS3 antibody
        • TMPS3_HUMAN antibody
        • Transmembrane protease serine 3 antibody
        • Tumor associated differentially expressed gene 12 protein antibody
        • Tumor-associated differentially-expressed gene 12 protein antibody
        • UNQ323/PRO382 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)

        ab167160 showing +ve staining in Human cervical carcinoma.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)

        ab167160 showing +ve staining in Human normal tonsil.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)

        ab167160 showing +ve staining in Human lung adenocarcinoma.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)

        ab167160 showing +ve staining in Human normal kidney.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)

        ab167160 showing +ve staining in Human normal colon.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)

        ab167160 showing +ve staining in Human breast carcinoma.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Western blot - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
        Western blot - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
        All lanes : Anti-TMPRSS3 antibody [EPR7048] (ab167160) at 1/1000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : PC-12 cell lysate
        Lane 3 : A431 cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 49 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPRSS3 antibody [EPR7048] (ab167160)

        Immunohistochemical analysis of paraffin embedded Human thyroid gland papillary carcinoma tissue labeling TMPRSS3 with ab167160 at a 1/100 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunocytochemistry/ Immunofluorescence - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
        Immunocytochemistry/ Immunofluorescence - Anti-TMPRSS3 antibody [EPR7048] (ab167160)
        Immunocytochemical analysis of A431 cells labeling TMPRSS3 with ab167160 at a 1/250 dilution.

      References

      ab167160 has not yet been referenced specifically in any publications.

      Publishing research using ab167160? Please let us know so that we can cite the reference in this datasheet.

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