Overview

  • Product name
  • Description
    Goat polyclonal to TMPRSS5
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide:

    LDWIHDTAQDSLL

    , corresponding to C terminal amino acids 445-457 of Human TMPRSS5

  • General notes
    Principal Names - TMPRSS5; transmembrane protease, serine 5 (spinesin); SPINESIN; spinesin. Official Gene Symbol - TMPRSS5. GenBank Accession Number – NP_110397. LocusLink ID - 80975

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.05% Tris, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 52 kDa.

Detects two bands of approximately 35 and 52 kDa in mouse brain lysate.

Target

  • Function
    May play a role in hearing.
  • Tissue specificity
    Brain-specific. Predominantly expressed in neurons, in their axons, and at the synapses of motoneurons in the spinal cord.
  • Involvement in disease
    Note=Defects in TMPRSS5 may be a cause of deafness.
  • Sequence similarities
    Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
    Contains 1 SRCR domain.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC141886 antibody
    • MGC148044 antibody
    • OTTHUMP00000238209 antibody
    • OTTHUMP00000238210 antibody
    • OTTHUMP00000238211 antibody
    • OTTHUMP00000238212 antibody
    • OTTHUMP00000238213 antibody
    • Spinesin antibody
    • TMPRSS 5 antibody
    • TMPRSS5 antibody
    • TMPS5_HUMAN antibody
    • Transmembrane protease serine 5 antibody
    • Transmembrane protease, serine 5 (spinesin) antibody
    • Transmembrane protease, serine 5 antibody
    see all

Images

  • ab2245 staining (2µg/ml) of Mouse Brain lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. ab2245 staining (2µg/ml) of Mouse Brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • ab2245 staining (0.5µg/ml) of Human Brain (Cerebral Cortex) lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

References

This product has been referenced in:
  • Zhang Y  et al. Paclitaxel inhibits breast cancer metastasis via suppression of Aurora kinase-mediated cofilin-1 activity. Exp Ther Med 15:1269-1276 (2018). Read more (PubMed: 29434713) »
  • Carmena M  et al. The chromosomal passenger complex activates polo kinase at centromeres. PLoS Biol 10:e1001250 (2012). IF . Read more (PubMed: 22291575) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your enquiry and your patience, the source of ab2245 has confirmed that Western blots were done using reducing conditions. The exact protocol is below: - SDS PAGE. Samples were run at 200V constant on a 12% acrylamide SDS-PAGE mini gel - using Biorad Mini-Protean 3 kit and protocols. Before loading samples had 5% (v/v) 2-ME added and were boiled for 3 minutes. I hope this information will help, please do not hesitate to contact us if you need further assistance,

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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