Product nameTMPyP4 tosylate, Telomerase inhibitor
DescriptionTelomerase inhibitor. Binds and stabilizes DNA G-quadruplexes.
Biological descriptionCationic porphyrin. Telomerase inhibitor (IC50 = 6.5 µM). Binds and stabilizes DNA G quadruplexes resulting in telomerase inhibition. Displays antitumor effect.
Chemical name5,10,15,20-Tetrakis(1-methylpyridinium-4-yl)porphyrin tetra(p-toluenesulfonate)
Storage instructionsStore at -20°C. It is important to note that this product is reported to be light sensitive. Store In the Dark. Store under desiccating conditions.
Solubility overviewSoluble in water to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Our Abpromise guarantee covers the use of ab120793 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab2893 staining ?H2A.X in weri cells treated with TMPyP4 tosylate (ab120793), by ICC/IF. Increase of ?H2A.X nuclear expression correlates with increased concentration of TMPyP4 tosylate, as described in literature.
The cells were incubated at 37°C for 24 hours in media containing different concentrations of ab120793 (TMPyP4 tosylate ) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab2893 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab120793 has been referenced in 2 publications.
- Díaz-Cartagena DC et al. Label-Free Telomerase Activity Detection via Electrochemical Impedance Spectroscopy. ACS Omega 4:16724-16732 (2019). PubMed: 31646217
- Cid-Samper F et al. An Integrative Study of Protein-RNA Condensates Identifies Scaffolding RNAs and Reveals Players in Fragile X-Associated Tremor/Ataxia Syndrome. Cell Rep 25:3422-3434.e7 (2018). PubMed: 30566867