Key features and details
- Assay type: Cell-based
- Detection method: Fluorescent
- Platform: Microplate reader, Fluor. microscope, Flow cyt.
- Sample type: Adherent cells, Suspension cells
TMRM Assay Kit (Mitochondrial Membrane Potential)
Sample typeAdherent cells, Suspension cells
TMRM Assay Kit (Mitochondrial Membrane Potential) ab228569 is designed to quantify changes in mitochondrial membrane potential in living cells using flow cytometry, fluorescence plate reader, or fluorescence microscopy. It uses the red-fluorescent probe TMRM (tetramethylrhodamine, methyl ester) that localizes in mitochondria and detects mitochondrial membrane depolarization.
Related assay kits
TMRE is the ethyl ester of tetramethylrhodamine and is a close equivalent of TMRM. TMRE is used about 3 times more than TMRM for mitochondrial membrane potential assays.
TMRE-Mitochondrial Membrane Potential Assay Kit ab113852 is our most popular assay kit for mitochondrial membrane potential analysis.
TMRM staining is used to monitor mitochondrial function. When accumulated in a negatively charge polarized mitochondria, TMRM fluoresces orange. When mitochondrial membrane potential collapses in apoptotic or metabolically stressed cells, the TMRM reagent is dispersed throughout the cell cytosol and fluorescence levels drop dramatically.
This kit provides enough reagents to analyze 5 x 96 wells (500 samples).
PlatformMicroplate reader, Fluor. microscope, Flow cyt.
Storage instructionsPlease refer to protocols.
Components 1 kit 5X Live Cell Imaging Buffer 1 x 30ml FCCP 1 x 25µl TMRM Reagent 1 x 100µl
ab228569 has not yet been referenced specifically in any publications.