TMRM Assay Kit (Mitochondrial Membrane Potential) (ab228569)
- Datasheet
- References
- Protocols
Overview
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Product name
TMRM Assay Kit (Mitochondrial Membrane Potential) -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based -
Product overview
TMRM Assay Kit (Mitochondrial Membrane Potential) ab228569 is designed to quantify changes in mitochondrial membrane potential in living cells using flow cytometry, fluorescence plate reader, or fluorescence microscopy. It uses the red-fluorescent probe TMRM (tetramethylrhodamine, methyl ester) that localizes in mitochondria and detects mitochondrial membrane depolarization.
Related assay kits
TMRE is the ethyl ester of tetramethylrhodamine and is a close equivalent of TMRM. TMRE is used about 3 times more than TMRM for mitochondrial membrane potential assays.
TMRE-Mitochondrial Membrane Potential Assay Kit ab113852 is our most popular assay kit for mitochondrial membrane potential analysis. -
Notes
TMRM staining is used to monitor mitochondrial function. When accumulated in a negatively charge polarized mitochondria, TMRM fluoresces orange. When mitochondrial membrane potential collapses in apoptotic or metabolically stressed cells, the TMRM reagent is dispersed throughout the cell cytosol and fluorescence levels drop dramatically.
This kit provides enough reagents to analyze 5 x 96 wells (500 samples).
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Platform
Microplate reader, Fluor. microscope, Flow cyt.
Properties
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Storage instructions
Please refer to protocols. -
Components 1 kit 5X Live Cell Imaging Buffer 1 x 30ml FCCP 1 x 25µl TMRM Reagent 1 x 100µl
Images
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Example dataHuman fibroblast cells stained using ab228569 TMRM Assay Kit (Mitochondrial Membrane Potential) (200 nM TMRM).
Protocols
References
ab228569 has not yet been referenced specifically in any publications.
