TMRM Assay Kit (Mitochondrial Membrane Potential)

(ab228569)

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Example data

    Key features and details

    • Assay type: Cell-based
    • Detection method: Fluorescent
    • Platform: Microplate reader, Fluor. microscope, Flow cyt.
    • Sample type: Adherent cells, Suspension cells

    Overview

    • Product name

      TMRM Assay Kit (Mitochondrial Membrane Potential)

    • Detection method

      Fluorescent

    • Sample type

      Adherent cells, Suspension cells

    • Assay type

      Cell-based

    • Product overview

      TMRM Assay Kit (Mitochondrial Membrane Potential) ab228569 is designed to quantify changes in mitochondrial membrane potential in living cells using flow cytometry, fluorescence plate reader, or fluorescence microscopy. It uses the red-fluorescent probe TMRM (tetramethylrhodamine, methyl ester) that localizes in mitochondria and detects mitochondrial membrane depolarization.


      Related assay kits
      TMRE is the ethyl ester of tetramethylrhodamine and is a close equivalent of TMRM. TMRE is used about 3 times more than TMRM for mitochondrial membrane potential assays.
      TMRE-Mitochondrial Membrane Potential Assay Kit ab113852 is our most popular assay kit for mitochondrial membrane potential analysis.

    • Notes

      TMRM staining is used to monitor mitochondrial function. When accumulated in a negatively charge polarized mitochondria, TMRM fluoresces orange. When mitochondrial membrane potential collapses in apoptotic or metabolically stressed cells, the TMRM reagent is dispersed throughout the cell cytosol and fluorescence levels drop dramatically.

      This kit provides enough reagents to analyze 5 x 96 wells (500 samples).

    • Platform

      Microplate reader, Fluor. microscope, Flow cyt.

    Properties

    • Storage instructions

      Please refer to protocols.
    • Components 1 kit
      5X Live Cell Imaging Buffer 1 x 30ml
      FCCP 1 x 25µl
      TMRM Reagent 1 x 100µl

    Images

    • Example data
      Example data

      Human fibroblast cells stained using ab228569 TMRM Assay Kit (Mitochondrial Membrane Potential) (200 nM TMRM).

    References (0)

    Publishing research using ab228569? Please let us know so that we can cite the reference in this datasheet.

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    Customer reviews and Q&As

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    Easy to use and good results

     Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    abreview image
    We used this kit to analyze MMP in HCT116 cells. The kit is easy to use, the set-up was fast and the results were robust.
    We initially planned a plate based read-out - which is why we ordered that kit - but ended up using flow cytometry as readout for which the TMRM alone would have been enough (ab275547) and a cheap alternative.
    The attached image shows the drop in MMP 30 min after FCCP administration.

    Abcam user community

    Verified customer

    Submitted Dec 02 2020

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