TMRM Assay Kit (Mitochondrial Membrane Potential) (ab228569)

Overview

  • Product name

    TMRM Assay Kit (Mitochondrial Membrane Potential)
  • Detection method

    Fluorescent
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based
  • Product overview

    TMRM Assay Kit (Mitochondrial Membrane Potential) ab228569 is designed to quantify changes in mitochondrial membrane potential in living cells using flow cytometry, fluorescence plate reader, or fluorescence microscopy. It uses the red-fluorescent probe TMRM (tetramethylrhodamine, methyl ester) that localizes in mitochondria and detects mitochondrial membrane depolarization.


    Related assay kits
    TMRE is the ethyl ester of tetramethylrhodamine and is a close equivalent of TMRM. TMRE is used about 3 times more than TMRM for mitochondrial membrane potential assays.
    TMRE-Mitochondrial Membrane Potential Assay Kit ab113852 is our most popular assay kit for mitochondrial membrane potential analysis.

  • Notes

    TMRM staining is used to monitor mitochondrial function. When accumulated in a negatively charge polarized mitochondria, TMRM fluoresces orange. When mitochondrial membrane potential collapses in apoptotic or metabolically stressed cells, the TMRM reagent is dispersed throughout the cell cytosol and fluorescence levels drop dramatically.

    This kit provides enough reagents to analyze 5 x 96 wells (500 samples).

  • Platform

    Microplate reader, Fluor. microscope, Flow cyt.

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 1 kit
    5X Live Cell Imaging Buffer 1 x 30ml
    FCCP 1 x 25µl
    TMRM Reagent 1 x 100µl

Images

  • Human fibroblast cells stained using ab228569 TMRM Assay Kit (Mitochondrial Membrane Potential) (200 nM TMRM).

Protocols

References

ab228569 has not yet been referenced specifically in any publications.

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