Overview

  • Product name
    Anti-TMS1 antibody [EPR10403]
    See all TMS1 primary antibodies
  • Description
    Rabbit monoclonal [EPR10403] to TMS1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TMS1 aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9ULZ3

  • Positive control
    • WB: THP-1 and U937 cell lysates. ICC/IF: HeLa and THP-1 cells. Flow Cyt: THP-1 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab155970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 22 kDa.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases.
    • Tissue specificity
      Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U937, but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and Molt 4 lymphocytic leukemia cells.
    • Sequence similarities
      Contains 1 CARD domain.
      Contains 1 DAPIN domain.
    • Domain
      Interacts with CIAS1/PYPAF1 and PYDC1 via the DAPIN domain.
    • Post-translational
      modifications
      Phosphorylated.
    • Cellular localization
      Cytoplasm. Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures.
    • Information by UniProt
    • Database links
    • Alternative names
      • Apoptosis associated speck like protein containing a CARD antibody
      • Apoptosis-associated speck-like protein containing a CARD antibody
      • ASC antibody
      • ASC_HUMAN antibody
      • CARD 5 antibody
      • CARD5 antibody
      • Caspase recruitment domain containing protein 5 antibody
      • Caspase recruitment domain protein 5 antibody
      • Caspase recruitment domain-containing protein 5 antibody
      • hASC antibody
      • MGC10332 antibody
      • PYCARD antibody
      • PYD and CARD domain containing antibody
      • PYD and CARD domain containing protein antibody
      • PYD and CARD domain-containing protein antibody
      • Target of methylation induced silencing 1 antibody
      • Target of methylation-induced silencing 1 antibody
      • TMS 1 antibody
      • TMS antibody
      • TMS1 antibody
      see all

    Images

    • All lanes : Anti-TMS1 antibody [EPR10403] (ab155970) at 1/5000 dilution

      Lane 1 : THP-1 (Human monocytic leukemia cell line) whole cell lysate
      Lane 2 : U937 (Human histiocytic lymphoma cell line) whole cell lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 20000 µg

      Predicted band size: 22 kDa



      Blocking and diluting buffer: 5% NFDM/TBST

    • All lanes : Anti-TMS1 antibody [EPR10403] (ab155970) at 1/1000 dilution

      Lane 1 : THP1 cell lysate
      Lane 2 : U937 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 22 kDa

    • ab133645 staining TMS1 in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence).

      Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/250. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab195889 was used as a counterstain for primary antibody ab155970 at 1/200. DAPI was used as a nuclear counterstain and PBS as a negative control.

    • ab155970 staining TMS1 in the human cell line THP-1 (Human monocytic leukemia cell line) by flow cytometry.

      Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/50. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

      Isoytype control: Rabbit monoclonal IgG (Black)

      Unlabeled control: Cell without incubation with primary antibody and secondary antibody (Blue)

    • Immunofluorescence analysis of THP-1 (Human monocytic leukemia cell line) cells, labeling TMS1 using ab155970 at a 1/100 dilution.

    References

    This product has been referenced in:
    • Guo Q  et al. Cytokine Secretion and Pyroptosis of Thyroid Follicular Cells Mediated by Enhanced NLRP3, NLRP1, NLRC4, and AIM2 Inflammasomes Are Associated With Autoimmune Thyroiditis. Front Immunol 9:1197 (2018). Read more (PubMed: 29915579) »
    • Cao S  et al. Melatonin-mediated mitophagy protects against early brain injury after subarachnoid hemorrhage through inhibition of NLRP3 inflammasome activation. Sci Rep 7:2417 (2017). WB . Read more (PubMed: 28546552) »
    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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