Anti-TMS1/ASC antibody (ab111852)
Key features and details
- Goat polyclonal to TMS1/ASC
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TMS1/ASC antibody
See all TMS1/ASC primary antibodies -
Description
Goat polyclonal to TMS1/ASC -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human TMS1/ASC aa 150-250 (C terminal).
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Positive control
- Human Liver and tonsil tissues; HeLa lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab111852 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 22 kDa.
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IHC-P |
Use a concentration of 2.5 µg/ml.
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Notes |
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WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 22 kDa. |
IHC-P
Use a concentration of 2.5 µg/ml. |
Target
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Function
Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. -
Tissue specificity
Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U937, but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and Molt 4 lymphocytic leukemia cells. -
Sequence similarities
Contains 1 CARD domain.
Contains 1 DAPIN domain. -
Domain
Interacts with CIAS1/PYPAF1 and PYDC1 via the DAPIN domain. -
Post-translational
modificationsPhosphorylated. -
Cellular localization
Cytoplasm. Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures. - Information by UniProt
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Database links
- Entrez Gene: 29108 Human
- Omim: 606838 Human
- SwissProt: Q9ULZ3 Human
- Unigene: 499094 Human
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Alternative names
- Apoptosis associated speck like protein containing a CARD antibody
- Apoptosis-associated speck-like protein containing a CARD antibody
- ASC antibody
see all
Images
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ab111852, at 2.5µg/ml, staining TMS1/ASC in Formalin-fixed, Paraffin-embedded Human Liver tissue by Immunohistochemistry.
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ab111852, at 2.5µg/ml, staining TMS1/ASC in Formalin-fixed, Paraffin-embedded Human Tonsil tissue by Immunohistochemistry.
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Anti-TMS1/ASC antibody (ab111852) at 1 µg/ml + HeLa lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 22 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab111852 has not yet been referenced specifically in any publications.