Anti-TMS1/ASC antibody (ab175449)
Key features and details
- Goat polyclonal to TMS1/ASC
- Suitable for: ICC
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-TMS1/ASC antibody
See all TMS1/ASC primary antibodies -
Description
Goat polyclonal to TMS1/ASC -
Host species
Goat -
Tested applications
Suitable for: ICCmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Cow, Dog, Pig
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Immunogen
Synthetic peptide corresponding to Mouse TMS1/ASC aa 51-63 (internal sequence) (Cysteine residue). NP_075747.3.
Sequence:DLTDKLVSYYLES
Database link: Q9EPB4 -
Positive control
- ICC: NIH3T3 and KNRK cells.
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General notes
This product was previously labelled as TMS1
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab175449 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175449 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
Use a concentration of 10 µg/ml.
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| Notes |
|---|
|
ICC
Use a concentration of 10 µg/ml. |
Target
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Function
Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. -
Tissue specificity
Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U937, but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and Molt 4 lymphocytic leukemia cells. -
Sequence similarities
Contains 1 CARD domain.
Contains 1 DAPIN domain. -
Domain
Interacts with CIAS1/PYPAF1 and PYDC1 via the DAPIN domain. -
Post-translational
modificationsPhosphorylated. -
Cellular localization
Cytoplasm. Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures. - Information by UniProt
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Database links
- Entrez Gene: 282846 Cow
- Entrez Gene: 282817 Rat
- SwissProt: Q8HXK9 Cow
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Alternative names
- Apoptosis associated speck like protein containing a CARD antibody
- Apoptosis-associated speck-like protein containing a CARD antibody
- ASC antibody
see all
Images
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Immunocytochemistry - Anti-TMS1/ASC antibody (ab175449)
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Immunocytochemistry - Anti-TMS1/ASC antibody (ab175449)Immunofluorescence analysis of paraformaldehyde fixed KNRK cells labelling TMS1/ASC cells with ab175449 at 10 µg/mL. Cells were fixed with 0.15% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 10 µg/mL used as the secondary antibody (green). Nuclear DNA was labelled with DAPI (blue). Negative control: Unimmunized goat IgG 10 µg/mL.
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab175449 has been referenced in 11 publications.
- Fu L et al. Exogenous carbon monoxide protects against mitochondrial DNA-induced hippocampal pyroptosis in a model of hemorrhagic shock and resuscitation. Int J Mol Med 45:1176-1186 (2020). PubMed: 32124959
- Hang L et al. Ox-LDL Causes Endothelial Cell Injury Through ASK1/NLRP3-Mediated Inflammasome Activation via Endoplasmic Reticulum Stress. Drug Des Devel Ther 14:731-744 (2020). PubMed: 32158192
- Wang M et al. trans-Cinnamaldehyde Reverses Depressive-Like Behaviors in Chronic Unpredictable Mild Stress Rats by Inhibiting NF-?B/NLRP3 Inflammasome Pathway. Evid Based Complement Alternat Med 2020:4572185 (2020). PubMed: 32328132
- Gao Y et al. Role of Parkin-mediated mitophagy in the protective effect of polydatin in sepsis-induced acute kidney injury. J Transl Med 18:114 (2020). PubMed: 32131850
- Dai G et al. Caffeic Acid Phenethyl Ester Prevents Colitis-Associated Cancer by Inhibiting NLRP3 Inflammasome. Front Oncol 10:721 (2020). PubMed: 32435622