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Cell Biology Apoptosis Intracellular Associated Proteins
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Anti-TMS1/ASC antibody (ab175449)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (11)

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Immunocytochemistry - Anti-TMS1/ASC antibody (ab175449)
  • Immunocytochemistry - Anti-TMS1/ASC antibody (ab175449)

Key features and details

  • Goat polyclonal to TMS1/ASC
  • Suitable for: ICC
  • Reacts with: Rat
  • Isotype: IgG

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Overview

  • Product name

    Anti-TMS1/ASC antibody
    See all TMS1/ASC primary antibodies
  • Description

    Goat polyclonal to TMS1/ASC
  • Host species

    Goat
  • Tested applications

    Suitable for: ICCmore details
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Mouse TMS1/ASC aa 51-63 (internal sequence) (Cysteine residue). NP_075747.3.
    Sequence:

    DLTDKLVSYYLES


    Database link: Q9EPB4
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • ICC: NIH3T3 and KNRK cells.
  • General notes

     This product was previously labelled as TMS1

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 99% Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab175449 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Associated Proteins
    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • CARD Family
    • Cancer
    • Tumor biomarkers
    • Other

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Positive Controls

    • Mouse spleen tissue lysate - total protein (ab29293)
    • Mouse spleen tissue lysate - total protein (ab7937)
  • Recombinant Protein

    • Recombinant Human TMS1/ASC protein (ab131786)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab175449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Use a concentration of 10 µg/ml.
Notes
ICC
Use a concentration of 10 µg/ml.

Target

  • Function

    Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases.
  • Tissue specificity

    Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U937, but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and Molt 4 lymphocytic leukemia cells.
  • Sequence similarities

    Contains 1 CARD domain.
    Contains 1 DAPIN domain.
  • Domain

    Interacts with CIAS1/PYPAF1 and PYDC1 via the DAPIN domain.
  • Post-translational
    modifications

    Phosphorylated.
  • Cellular localization

    Cytoplasm. Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures.
  • Target information above from: UniProt accession Q9ULZ3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 282846 Cow
    • Entrez Gene: 282817 Rat
    • SwissProt: Q8HXK9 Cow
    • Alternative names

      • Apoptosis associated speck like protein containing a CARD antibody
      • Apoptosis-associated speck-like protein containing a CARD antibody
      • ASC antibody
      • ASC_HUMAN antibody
      • CARD 5 antibody
      • CARD5 antibody
      • Caspase recruitment domain containing protein 5 antibody
      • Caspase recruitment domain protein 5 antibody
      • Caspase recruitment domain-containing protein 5 antibody
      • hASC antibody
      • MGC10332 antibody
      • PYCARD antibody
      • PYD and CARD domain containing antibody
      • PYD and CARD domain containing protein antibody
      • PYD and CARD domain-containing protein antibody
      • Target of methylation induced silencing 1 antibody
      • Target of methylation-induced silencing 1 antibody
      • TMS 1 antibody
      • TMS antibody
      • TMS1 antibody
      see all

    Images

    • Immunocytochemistry - Anti-TMS1/ASC antibody (ab175449)
      Immunocytochemistry - Anti-TMS1/ASC antibody (ab175449)
    • Immunocytochemistry - Anti-TMS1/ASC antibody (ab175449)
      Immunocytochemistry - Anti-TMS1/ASC antibody (ab175449)

      Immunofluorescence analysis of paraformaldehyde fixed KNRK cells labelling TMS1/ASC cells with ab175449 at 10 µg/mL. Cells were fixed with 0.15% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 10 µg/mL used as the secondary antibody (green). Nuclear DNA was labelled with DAPI (blue).  Negative control: Unimmunized goat IgG 10 µg/mL.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (11)

    Publishing research using ab175449? Please let us know so that we can cite the reference in this datasheet.

    ab175449 has been referenced in 11 publications.

    • Fu L  et al. Exogenous carbon monoxide protects against mitochondrial DNA-induced hippocampal pyroptosis in a model of hemorrhagic shock and resuscitation. Int J Mol Med 45:1176-1186 (2020). PubMed: 32124959
    • Hang L  et al. Ox-LDL Causes Endothelial Cell Injury Through ASK1/NLRP3-Mediated Inflammasome Activation via Endoplasmic Reticulum Stress. Drug Des Devel Ther 14:731-744 (2020). PubMed: 32158192
    • Wang M  et al. trans-Cinnamaldehyde Reverses Depressive-Like Behaviors in Chronic Unpredictable Mild Stress Rats by Inhibiting NF-?B/NLRP3 Inflammasome Pathway. Evid Based Complement Alternat Med 2020:4572185 (2020). PubMed: 32328132
    • Gao Y  et al. Role of Parkin-mediated mitophagy in the protective effect of polydatin in sepsis-induced acute kidney injury. J Transl Med 18:114 (2020). PubMed: 32131850
    • Dai G  et al. Caffeic Acid Phenethyl Ester Prevents Colitis-Associated Cancer by Inhibiting NLRP3 Inflammasome. Front Oncol 10:721 (2020). PubMed: 32435622
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TMS1/ASC antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.8
    Permeabilization
    No
    Specification
    liver
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 37°C
    Fixative
    Formaldehyde
    Read More

    durgesh dwivedi

    Verified customer

    Submitted Jul 16 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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