Anti-TMS1/ASC antibody (ab175449)
- Datasheet
- References (5)
- Protocols
Overview
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Product name
Anti-TMS1/ASC antibody
See all TMS1/ASC primary antibodies -
Description
Goat polyclonal to TMS1/ASC -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Cow, Dog, Human, Pig
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Immunogen
Synthetic peptide corresponding to Mouse TMS1/ASC aa 51-63 (internal sequence) (Cysteine residue). NP_075747.3.
Sequence:DLTDKLVSYYLES
Database link: Q9EPB4 -
Positive control
- Mouse and rat spleen lysates.
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General notes
This product was previously labelled as TMS1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab175449 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab175449 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 21 kDa. We recommend blocking in milk (3% milk in TBST) for 1 hour at room temperature. |
Target
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Function
Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. -
Tissue specificity
Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U937, but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and Molt 4 lymphocytic leukemia cells. -
Sequence similarities
Contains 1 CARD domain.
Contains 1 DAPIN domain. -
Domain
Interacts with CIAS1/PYPAF1 and PYDC1 via the DAPIN domain. -
Post-translational
modificationsPhosphorylated. -
Cellular localization
Cytoplasm. Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures. - Information by UniProt
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Database links
- Entrez Gene: 282846 Cow
- Entrez Gene: 29108 Human
- Entrez Gene: 66824 Mouse
- Entrez Gene: 282817 Rat
- Omim: 606838 Human
- SwissProt: Q8HXK9 Cow
- SwissProt: Q9ULZ3 Human
- SwissProt: Q9EPB4 Mouse
see all -
Alternative names
- Apoptosis associated speck like protein containing a CARD antibody
- Apoptosis-associated speck-like protein containing a CARD antibody
- ASC antibody
see all
Images
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Western blot - Anti-TMS1/ASC antibody (ab175449)All lanes : Anti-TMS1/ASC antibody (ab175449) at 3 µg/ml
Lane 1 : Mouse spleen lysates
Lane 2 : Rat spleen lysates
Lysates/proteins at 35 µg per lane.
Predicted band size: 21 kDaPrimary incubation was 1 hour. Detected by chemiluminescence.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Dang R et al. Predictable chronic mild stress promotes recovery from LPS-induced depression. Mol Brain 12:42 (2019). Read more (PubMed: 31053149) »
- Huang J et al. Development of benzoxazole deoxybenzoin oxime and acyloxylamine derivatives targeting innate immune sensors and xanthine oxidase for treatment of gout. Bioorg Med Chem 26:1653-1664 (2018). WB ; Rat . Read more (PubMed: 29472126) »
