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  1. Link

    tms1asc-antibody-ab175449.pdf

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Cell Biology Apoptosis Intracellular Associated Proteins
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Anti-TMS1/ASC antibody (ab175449)

  • Datasheet
Reviews (1) Submit a question References (25)

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Immunocytochemistry/ Immunofluorescence - Anti-TMS1/ASC antibody (ab175449)
  • Immunocytochemistry/ Immunofluorescence - Anti-TMS1/ASC antibody (ab175449)
  • Immunocytochemistry/ Immunofluorescence - Anti-TMS1/ASC antibody (ab175449)

Key features and details

  • Goat polyclonal to TMS1/ASC
  • Suitable for: ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-TMS1/ASC antibody [EPR10403] (ab155970)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-TMS1/ASC antibody
    See all TMS1/ASC primary antibodies
  • Description

    Goat polyclonal to TMS1/ASC
  • Host species

    Goat
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Mouse TMS1/ASC aa 51-63 (internal sequence) (Cysteine residue). NP_075747.3.
    Sequence:

    DLTDKLVSYYLES


    Database link: Q9EPB4
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • ICC/IF: NIH3T3 cells, KNRK cells and HeLa cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 99% Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab175449 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Associated Proteins
    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • CARD Family
    • Cancer
    • Tumor biomarkers
    • Other
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Positive Controls

    • Mouse spleen tissue lysate - total protein (ab29293)
    • Mouse spleen tissue lysate - total protein (ab7937)
  • Recombinant Protein

    • Recombinant Human TMS1/ASC protein (ab131786)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab175449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use a concentration of 10 µg/ml.
Notes
ICC/IF
Use a concentration of 10 µg/ml.

Target

  • Function

    Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases.
  • Tissue specificity

    Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U937, but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and Molt 4 lymphocytic leukemia cells.
  • Sequence similarities

    Contains 1 CARD domain.
    Contains 1 DAPIN domain.
  • Domain

    Interacts with CIAS1/PYPAF1 and PYDC1 via the DAPIN domain.
  • Post-translational
    modifications

    Phosphorylated.
  • Cellular localization

    Cytoplasm. Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures.
  • Target information above from: UniProt accession Q9ULZ3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 282846 Cow
    • Entrez Gene: 29108 Human
    • Entrez Gene: 66824 Mouse
    • Entrez Gene: 282817 Rat
    • Omim: 606838 Human
    • SwissProt: Q8HXK9 Cow
    • SwissProt: Q9ULZ3 Human
    • SwissProt: Q9EPB4 Mouse
    • Unigene: 499094 Human
    • Unigene: 24163 Mouse
    see all
  • Alternative names

    • Apoptosis associated speck like protein containing a CARD antibody
    • Apoptosis-associated speck-like protein containing a CARD antibody
    • ASC antibody
    • ASC_HUMAN antibody
    • CARD 5 antibody
    • CARD5 antibody
    • Caspase recruitment domain containing protein 5 antibody
    • Caspase recruitment domain protein 5 antibody
    • Caspase recruitment domain-containing protein 5 antibody
    • hASC antibody
    • MGC10332 antibody
    • PYCARD antibody
    • PYD and CARD domain containing antibody
    • PYD and CARD domain containing protein antibody
    • PYD and CARD domain-containing protein antibody
    • Target of methylation induced silencing 1 antibody
    • Target of methylation-induced silencing 1 antibody
    • TMS 1 antibody
    • TMS antibody
    • TMS1 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-TMS1/ASC antibody (ab175449)
    Immunocytochemistry/ Immunofluorescence - Anti-TMS1/ASC antibody (ab175449)

    Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation with ab175449 for 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

  • Immunocytochemistry/ Immunofluorescence - Anti-TMS1/ASC antibody (ab175449)
    Immunocytochemistry/ Immunofluorescence - Anti-TMS1/ASC antibody (ab175449)
    Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation with ab175449 for 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • Immunocytochemistry/ Immunofluorescence - Anti-TMS1/ASC antibody (ab175449)
    Immunocytochemistry/ Immunofluorescence - Anti-TMS1/ASC antibody (ab175449)

    Immunofluorescence analysis of paraformaldehyde fixed KNRK cells, permeabilized with 0.15% Triton. Primary incubation with ab175449 for 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (25)

Publishing research using ab175449? Please let us know so that we can cite the reference in this datasheet.

ab175449 has been referenced in 25 publications.

  • Mo G  et al. IP3R1 regulates Ca2+ transport and pyroptosis through the NLRP3/Caspase-1 pathway in myocardial ischemia/reperfusion injury. Cell Death Discov 7:31 (2021). PubMed: 33568649
  • Li S  et al. Knockdown of dual oxidase 1 suppresses activin A-induced fibrosis in cardiomyocytes via the reactive oxygen species-dependent pyroptotic pathway. Int J Biochem Cell Biol 131:105902 (2021). PubMed: 33309621
  • Chen X  et al. GLP-1 alleviates NLRP3 inflammasome-dependent inflammation in perivascular adipose tissue by inhibiting the NF-?B signalling pathway. J Int Med Res 49:300060521992981 (2021). PubMed: 33641439
  • Qin ZY  et al. Toll-like receptor 4 activates the NLRP3 inflammasome pathway and periodontal inflammaging by inhibiting Bmi-1 expression. Int J Mol Med 47:137-150 (2021). PubMed: 33236134
  • Tao Z  et al. Xi Lei San Attenuates Dextran Sulfate Sodium-Induced Colitis in Rats and TNF-a-Stimulated Colitis in CACO2 Cells: Involvement of the NLRP3 Inflammasome and Autophagy. Mediators Inflamm 2021:1610251 (2021). PubMed: 33967625
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TMS1/ASC antibody

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.8
Permeabilization
No
Specification
liver
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 37°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

durgesh dwivedi

Verified customer

Submitted Jul 16 2018

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