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    tms1asc-antibody-ab2236.pdf

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Cell Biology Apoptosis Intracellular Associated Proteins
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Anti-TMS1/ASC antibody (ab2236)

  • Datasheet
  • SDS
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Western blot - Anti-TMS1/ASC antibody (ab2236)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody (ab2236)

Key features and details

  • Goat polyclonal to TMS1/ASC
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human TMS1/ASC protein (ab131786)
Secondary
Product image
Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)

View more associated products

Overview

  • Product name

    Anti-TMS1/ASC antibody
    See all TMS1/ASC primary antibodies
  • Description

    Goat polyclonal to TMS1/ASC
  • Host species

    Goat
  • Specificity

    This antibody is expected to recognize all three reported isforms (NP_037390; NP_660183; NP_660184).
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human TMS1/ASC aa 182-195.
    Sequence:

    C-RESQSYLVEDLERS


    (Peptide available as ab22875, ab8395)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • HeLa cell lysate Human Liver
  • General notes

     This product was previously labelled as TMS1

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Associated Proteins
    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • CARD Family
    • Cancer
    • Tumor biomarkers
    • Other

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant Human TMS1/ASC protein (ab131786)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
Application Abreviews Notes
IHC-P
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 0.2 - 0.6 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).

1 hour primary incubation is recommended for this product.

Notes
IHC-P
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 0.2 - 0.6 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).

1 hour primary incubation is recommended for this product.

Target

  • Function

    Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases.
  • Tissue specificity

    Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U937, but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and Molt 4 lymphocytic leukemia cells.
  • Sequence similarities

    Contains 1 CARD domain.
    Contains 1 DAPIN domain.
  • Domain

    Interacts with CIAS1/PYPAF1 and PYDC1 via the DAPIN domain.
  • Post-translational
    modifications

    Phosphorylated.
  • Cellular localization

    Cytoplasm. Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures.
  • Target information above from: UniProt accession Q9ULZ3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 29108 Human
    • Omim: 606838 Human
    • SwissProt: Q9ULZ3 Human
    • Unigene: 499094 Human
    • Alternative names

      • Apoptosis associated speck like protein containing a CARD antibody
      • Apoptosis-associated speck-like protein containing a CARD antibody
      • ASC antibody
      • ASC_HUMAN antibody
      • CARD 5 antibody
      • CARD5 antibody
      • Caspase recruitment domain containing protein 5 antibody
      • Caspase recruitment domain protein 5 antibody
      • Caspase recruitment domain-containing protein 5 antibody
      • hASC antibody
      • MGC10332 antibody
      • PYCARD antibody
      • PYD and CARD domain containing antibody
      • PYD and CARD domain containing protein antibody
      • PYD and CARD domain-containing protein antibody
      • Target of methylation induced silencing 1 antibody
      • Target of methylation-induced silencing 1 antibody
      • TMS 1 antibody
      • TMS antibody
      • TMS1 antibody
      see all

    Images

    • Western blot - Anti-TMS1/ASC antibody (ab2236)
      Western blot - Anti-TMS1/ASC antibody (ab2236)
      Anti-TMS1/ASC antibody (ab2236) at 0.2 µg/ml + HeLa lysate

      Developed using the ECL technique.

      Predicted band size: 22 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody (ab2236)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody (ab2236)

      Immunohistochemical analysis of human liver tissue staining TMS1/ASC with ab2236 at 4µg/ml. Steamed antigen retrieval with citrate buffer pH6, HRP-staining.

       

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab2236? Please let us know so that we can cite the reference in this datasheet.

    ab2236 has not yet been referenced specifically in any publications.

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