Anti-TMS1/ASC antibody (ab2236)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-TMS1/ASC antibody
See all TMS1/ASC primary antibodies -
Description
Goat polyclonal to TMS1/ASC -
Host species
Goat -
Specificity
This antibody is expected to recognize all three reported isforms (NP_037390; NP_660183; NP_660184). -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
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Positive control
- HeLa cell lysate Human Liver
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General notes
This product was previously labelled as TMS1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab2236 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use a concentration of 0.2 - 0.6 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). |
Target
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Function
Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. -
Tissue specificity
Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U937, but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and Molt 4 lymphocytic leukemia cells. -
Sequence similarities
Contains 1 CARD domain.
Contains 1 DAPIN domain. -
Domain
Interacts with CIAS1/PYPAF1 and PYDC1 via the DAPIN domain. -
Post-translational
modificationsPhosphorylated. -
Cellular localization
Cytoplasm. Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures. - Information by UniProt
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Database links
- Entrez Gene: 29108 Human
- Omim: 606838 Human
- SwissProt: Q9ULZ3 Human
- Unigene: 499094 Human
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Alternative names
- Apoptosis associated speck like protein containing a CARD antibody
- Apoptosis-associated speck-like protein containing a CARD antibody
- ASC antibody
see all
Images
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Western blot - Anti-TMS1/ASC antibody (ab2236)Anti-TMS1/ASC antibody (ab2236) at 0.2 µg/ml + HeLa lysate
Developed using the ECL technique.
Predicted band size: 22 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody (ab2236)Immunohistochemical analysis of human liver tissue staining TMS1/ASC with ab2236 at 4µg/ml. Steamed antigen retrieval with citrate buffer pH6, HRP-staining.
Datasheets and documents
References
ab2236 has not yet been referenced specifically in any publications.
