Key features and details
- Rabbit polyclonal to TMS1/ASC
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TMS1/ASC antibody
See all TMS1/ASC primary antibodies
DescriptionRabbit polyclonal to TMS1/ASC
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Rhesus monkey
Recombinant fragment within Human TMS1/ASC (internal sequence). The exact sequence is proprietary.
Database link: Q9ULZ3
- ICC/IF: HCT 116 cells. WB: 3xDDDDK-human TMS1/ASC-transfected HEK-293T whole cell lysate; THP-1 whole cell extract.
This product was previously labelled as TMS1
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227502 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/20000. Predicted molecular weight: 22 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionPromotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases.
Tissue specificityWidely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U937, but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and Molt 4 lymphocytic leukemia cells.
Sequence similaritiesContains 1 CARD domain.
Contains 1 DAPIN domain.
DomainInteracts with CIAS1/PYPAF1 and PYDC1 via the DAPIN domain.
Cellular localizationCytoplasm. Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures.
- Information by UniProt
- Apoptosis associated speck like protein containing a CARD antibody
- Apoptosis-associated speck-like protein containing a CARD antibody
- ASC antibody
2% paraformaldehyde-fixed HCT 116 (human colorectal carcinoma cell line) cells stained for TMS1/ASC (green) using ab227502 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
All lanes : Anti-TMS1/ASC antibody (ab227502) at 1/20000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : 3xDDDDK-human TMS1/ASC-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 22 kDa
12 % SDS-PAGE gel.
Anti-TMS1/ASC antibody (ab227502) at 1/500 dilution + THP-1 (human monocytic leukemia cell line) whole cell extract at 30 µg
Predicted band size: 22 kDa
12% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227502 has been referenced in 1 publication.
- Li J et al. LncRNA GAS5 suppresses ovarian cancer by inducing inflammasome formation. Biosci Rep N/A:N/A (2017). PubMed: 29229673