Overview

  • Product name

  • Description

    Rabbit polyclonal to TMS1/ASC
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Recombinant fragment within Human TMS1/ASC (internal sequence). The exact sequence is proprietary.
    Database link: Q9ULZ3

  • Positive control

    • ICC/IF: HCT 116 cells. WB: 3xDDDDK-human TMS1/ASC-transfected HEK-293T whole cell lysate; THP-1 whole cell extract.
  • General notes

     This product was previously labelled as TMS1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab227502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/20000. Predicted molecular weight: 22 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases.
  • Tissue specificity

    Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U937, but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and Molt 4 lymphocytic leukemia cells.
  • Sequence similarities

    Contains 1 CARD domain.
    Contains 1 DAPIN domain.
  • Domain

    Interacts with CIAS1/PYPAF1 and PYDC1 via the DAPIN domain.
  • Post-translational
    modifications

    Phosphorylated.
  • Cellular localization

    Cytoplasm. Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures.
  • Information by UniProt
  • Database links

  • Alternative names

    • Apoptosis associated speck like protein containing a CARD antibody
    • Apoptosis-associated speck-like protein containing a CARD antibody
    • ASC antibody
    • ASC_HUMAN antibody
    • CARD 5 antibody
    • CARD5 antibody
    • Caspase recruitment domain containing protein 5 antibody
    • Caspase recruitment domain protein 5 antibody
    • Caspase recruitment domain-containing protein 5 antibody
    • hASC antibody
    • MGC10332 antibody
    • PYCARD antibody
    • PYD and CARD domain containing antibody
    • PYD and CARD domain containing protein antibody
    • PYD and CARD domain-containing protein antibody
    • Target of methylation induced silencing 1 antibody
    • Target of methylation-induced silencing 1 antibody
    • TMS 1 antibody
    • TMS antibody
    • TMS1 antibody
    see all

Images

  • 2% paraformaldehyde-fixed HCT 116 (human colorectal carcinoma cell line) cells stained for TMS1/ASC (green) using ab227502 at 1/500 dilution in ICC/IF.

    Blue: Hoechst 33342 staining.

  • All lanes : Anti-TMS1/ASC antibody (ab227502) at 1/20000 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : 3xDDDDK-human TMS1/ASC-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 22 kDa



    12 % SDS-PAGE gel.

  • Anti-TMS1/ASC antibody (ab227502) at 1/500 dilution + THP-1 (human monocytic leukemia cell line) whole cell extract at 30 µg

    Predicted band size: 22 kDa



    12% SDS-PAGE gel.

References

This product has been referenced in:

See 1 Publication for this product

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